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Biological Magnetic Resonance Data Bank


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BMRB Entry 6438

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6438
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Title: Sequence-specific resonance assignments of the C-terminal, 137-residue pseudo-receiver domain of circadian input kinase (CikA) that resets the circadian clock in Synechococcus elongatus

Deposition date: 2004-12-17 Original release date: 2005-08-29

Authors: Gao, Tiyu; Zhang, Xiaofan; Xia, Youlin; Golden, Susan; LiWang, Andy

Citation: Gao, Tiyu; Zhang, Xiaofan; Xia, Youlin; Cho, Yoonsang; Sacchettini, James; Golden, Susan; LiWang, Andy. "1H, 13C and 15N Chemical Shift Assignments of the C-Terminal, 133-Residue Pseudo-Receiver Domain of Circadian Input Kinase (CikA) in Synechococcus elongatus"  J. Biomol. NMR 32, 259-259 (2005).

Assembly members:
CikA, polymer, 137 residues, 14772 Da.

Natural source:   Common Name: Synechococcus elongatus   Taxonomy ID: 32046   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechococcus elongatus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CikA: GSHMIDNLPAGHILLLEEED EAATVVCEMLTAAGFKVIWL VDGSTALDQLDLLQPIVILM AWPPPDQSCLLLLQHLREHQ ADPHPPLVLFLGEPPVDPLL TAQASAILSKPLDPQLLLTT LQGLCPPNLSEGDRPSS

Data sets:
Data typeCount
1H chemical shifts991
13C chemical shifts476
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CikA C-terminus1

Entities:

Entity 1, CikA C-terminus 137 residues - 14772 Da.

1   GLYSERHISMETILEASPASNLEUPROALA
2   GLYHISILELEULEULEUGLUGLUGLUASP
3   GLUALAALATHRVALVALCYSGLUMETLEU
4   THRALAALAGLYPHELYSVALILETRPLEU
5   VALASPGLYSERTHRALALEUASPGLNLEU
6   ASPLEULEUGLNPROILEVALILELEUMET
7   ALATRPPROPROPROASPGLNSERCYSLEU
8   LEULEULEUGLNHISLEUARGGLUHISGLN
9   ALAASPPROHISPROPROLEUVALLEUPHE
10   LEUGLYGLUPROPROVALASPPROLEULEU
11   THRALAGLNALASERALAILELEUSERLYS
12   PROLEUASPPROGLNLEULEULEUTHRTHR
13   LEUGLNGLYLEUCYSPROPROASNLEUSER
14   GLUGLYASPARGPROSERSER

Samples:

sample_CikAPsR: CikA, [U-99% 13C; U-99% 15N], 1.3 mM; NaCL 50 mM; Phosphate 20 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
CDCA(NCO)CAHAnot availablenot availablenot available
HCANnot availablenot availablenot available
HCA(CO)Nnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
(HB)CB(CGCDCE)HEnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
4D 13C-13C edited NOESYnot availablenot availablenot available

Software:

NMRPipe v2004.126.16.02 - NMR data processing

STAPP v4.2.6 - Spin system creation, partial sequence specific assignments

PIPP v4.2.6 - Spectra visualization

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAD79072
GB AAF82192 ABB56676 AJD58780
REF WP_011243194 YP_171592 YP_399663

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
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