BMRB Entry 6286
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6286
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Title: 1H and 15N assignment of SMRT DAD PubMed: 15837933
Deposition date: 2004-08-10 Original release date: 2005-06-02
Authors: Codina, Anna; Schwabe, John
Citation: Codina, Anna; Love, James; Li, Y.; Lazar, M.; Neuhaus, David; Schwabe, John. "From The Cover: Structural insights into the interaction and activation of histone deacetylase 3 by nuclear receptor corepressors." Proc. Natl. Acad. Sci. U. S. A. 102, 6009-6014 (2005).
Assembly members:
SMRT deacetylase activation domain, polymer, 68 residues, 8291.9 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SMRT deacetylase activation domain: NGLMADPMKVYKDRQVMNMW
SEQEKETFREKFMQHPKNFG
LIASFLERKTVAECVLYYYL
TKKNENYK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 403 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SMRT DAD | 1 |
Entities:
Entity 1, SMRT DAD 68 residues - 8291.9 Da.
| 1 | ASN | GLY | LEU | MET | ALA | ASP | PRO | MET | LYS | VAL | ||||
| 2 | TYR | LYS | ASP | ARG | GLN | VAL | MET | ASN | MET | TRP | ||||
| 3 | SER | GLU | GLN | GLU | LYS | GLU | THR | PHE | ARG | GLU | ||||
| 4 | LYS | PHE | MET | GLN | HIS | PRO | LYS | ASN | PHE | GLY | ||||
| 5 | LEU | ILE | ALA | SER | PHE | LEU | GLU | ARG | LYS | THR | ||||
| 6 | VAL | ALA | GLU | CYS | VAL | LEU | TYR | TYR | TYR | LEU | ||||
| 7 | THR | LYS | LYS | ASN | GLU | ASN | TYR | LYS |
Samples:
sample_1: SMRT deacetylase activation domain1 – 2 mM; NaCl 50 mM; sodium phosphate 50 mM
sample_2: SMRT deacetylase activation domain, [U-15N], 1 – 2 mM; NaCl 50 mM; sodium phosphate 50 mM
cond_1: ionic strength: 0.350 M; pH: 6.8; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| (1H, 1H) DQF-COSY | not available | not available | cond_1 |
| (1H, 1H) DQ-correlation | not available | not available | cond_1 |
| (1H, 1H) NOESY | not available | not available | cond_1 |
| (1H, 1H) TOCSY | not available | not available | cond_1 |
| 2D 15N-HSQC | not available | not available | cond_1 |
| 3D 15N-NOESY-HSQC | not available | not available | cond_1 |
| 3D 15N-TOCSY-HSQC | not available | not available | cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker DMX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| GB | AAB91446 |
| REF | XP_001102940 XP_004310483 XP_006751297 XP_006938465 XP_009180278 |
| TPG | DAA20733 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts