BMRB Entry 6059
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6059
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Title: Ras-induced losing of interaction between C1 and RBD domains of Nore1 PubMed: 16698549
Deposition date: 2004-01-02 Original release date: 2008-07-01
Authors: Guiberman, Elena; Wohlgemuth, Sabine; Herrmann, Christian; Harjes, Stefan; Muller, Karl-Heinz; Bayer, Peter
Citation: Harjes, Elena; Harjes, Stefan; Wohlgemuth, Sabine; Muller, Karl-Heinz; Krieger, Elmar; Herrmann, Christian; Bayer, Peter. "GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1" Structure 14, 881-888 (2006).
Assembly members:
putative Ras effector, polymer, 59 residues, 8255 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
putative Ras effector: PRVLAERGEGHRFVELALRG
GPGWCDLCGREVLRQALRCA
NCKFTCHSECRSLIQLDCR
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 110 |
| 1H chemical shifts | 329 |
| 15N chemical shifts | 55 |
| coupling constants | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C1 domain, Nore1 | 1 |
| 2 | ZINC ION (2+), 1 | 2 |
| 3 | ZINC ION (2+), 2 | 2 |
Entities:
Entity 1, C1 domain, Nore1 59 residues - 8255 Da.
| 1 | PRO | ARG | VAL | LEU | ALA | GLU | ARG | GLY | GLU | GLY | ||||
| 2 | HIS | ARG | PHE | VAL | GLU | LEU | ALA | LEU | ARG | GLY | ||||
| 3 | GLY | PRO | GLY | TRP | CYS | ASP | LEU | CYS | GLY | ARG | ||||
| 4 | GLU | VAL | LEU | ARG | GLN | ALA | LEU | ARG | CYS | ALA | ||||
| 5 | ASN | CYS | LYS | PHE | THR | CYS | HIS | SER | GLU | CYS | ||||
| 6 | ARG | SER | LEU | ILE | GLN | LEU | ASP | CYS | ARG |
Entity 2, ZINC ION (2+), 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: putative Ras effector, [U-95% 13C; U-90% 15N], 2.0 mM; pottasium phosphate puffer 20.0 mM; NaN3 0.5 mM; DTE 4 mM
condition_1: pH: 6.9; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | sample_1 | not available | condition_1 |
| 1H-15N NOESY | sample_1 | not available | condition_1 |
| CCONH | sample_1 | not available | condition_1 |
| HCCONH | sample_1 | not available | condition_1 |
| 1H-N15 TOCSY | sample_1 | not available | condition_1 |
| HCCH-COSY | sample_1 | not available | condition_1 |
Software:
Aurelia v2.8.11 - Visualisation of NMR-Spectra
NMR spectrometers:
- INOVA Varian 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE33312 |
| GB | AAC08580 AAH89605 EDL39713 EDL39714 |
| REF | NP_061220 |
| SP | Q5EBH1 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts