BMRB Entry 5991
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5991
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Title: Backbone and Sidechain 1H, 13C, and 15N Chemical Shift Assignments and Backbone 15N Relaxation Parameters for Murine Ets-1 deltaN301 PubMed: 15591056
Deposition date: 2003-10-31 Original release date: 2011-08-11
Authors: Lee, Gregory; Donaldson, Logan; Pufall, Miles; Kang, Hyun-Seo; Gentile, Lisa; Pot, Isabelle; Graves, Barbara; McIntosh, Lawrence
Citation: Lee, Gregory; Donaldson, Logan; Pufall, Miles; Kang, Hyun-Seo; Pot, Isabelle; Graves, Barbara; McIntosh, Lawrence. "The structural and dynamic basis of Ets-1 DNA Binding autoinhibition" J. Biol. Chem. 280, 7088-7099 (2005).
Assembly members:
Ets-1, polymer, 140 residues, 16226.51 Da.
Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Ets-1: KGTFKDYVRDRADLNKDKPV
IPAAALAGYTGSGPIQLWQF
LLELLTDKSCQSFISWTGDG
WEFKLSDPDEVARRWGKRKN
KPKMNYEKLSRGLRYYYDKN
IIHKTAGKRYVYRFVSDLQS
LLGYTPEELHAMLDVKPDAD
- assigned_chemical_shifts
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
- order_parameters
| Data type | Count |
| 1H chemical shifts | 1025 |
| 13C chemical shifts | 657 |
| 15N chemical shifts | 156 |
| T1 relaxation values | 125 |
| T2 relaxation values | 125 |
| order parameters | 124 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ets-1 DN301 monomer | 1 |
Entities:
Entity 1, Ets-1 DN301 monomer 140 residues - 16226.51 Da.
| 1 | LYS | GLY | THR | PHE | LYS | ASP | TYR | VAL | ARG | ASP | |
| 2 | ARG | ALA | ASP | LEU | ASN | LYS | ASP | LYS | PRO | VAL | |
| 3 | ILE | PRO | ALA | ALA | ALA | LEU | ALA | GLY | TYR | THR | |
| 4 | GLY | SER | GLY | PRO | ILE | GLN | LEU | TRP | GLN | PHE | |
| 5 | LEU | LEU | GLU | LEU | LEU | THR | ASP | LYS | SER | CYS | |
| 6 | GLN | SER | PHE | ILE | SER | TRP | THR | GLY | ASP | GLY | |
| 7 | TRP | GLU | PHE | LYS | LEU | SER | ASP | PRO | ASP | GLU | |
| 8 | VAL | ALA | ARG | ARG | TRP | GLY | LYS | ARG | LYS | ASN | |
| 9 | LYS | PRO | LYS | MET | ASN | TYR | GLU | LYS | LEU | SER | |
| 10 | ARG | GLY | LEU | ARG | TYR | TYR | TYR | ASP | LYS | ASN | |
| 11 | ILE | ILE | HIS | LYS | THR | ALA | GLY | LYS | ARG | TYR | |
| 12 | VAL | TYR | ARG | PHE | VAL | SER | ASP | LEU | GLN | SER | |
| 13 | LEU | LEU | GLY | TYR | THR | PRO | GLU | GLU | LEU | HIS | |
| 14 | ALA | MET | LEU | ASP | VAL | LYS | PRO | ASP | ALA | ASP |
Samples:
sample_1: Ets-1, [U-99% 15N; U-99% 13C], 0.3 – 0.8 mM; phosphate buffer 20 mM; NaCl 500 mM; DTT 5 mM; NaN3 0.02%
sample_2: Ets-1, [U-99% 15N], 0.3 – 0.8 mM
sample_3: Ets-1, [U-98% 15N; U-98% 13C; U-97% 2H], 0.3 – 0.8 mM
sample_4: Ets-1, [U-99% 15N; U-10% 13C], 0.3 – 0.8 mM
Condition_1: pH: 6.5; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C CT HSQC | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NNH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| HACAN | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCC-TOCSY | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| HNCOCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| EZ-HMQC-NH2 | not available | not available | not available |
| methyl CN NOESY | not available | not available | not available |
| simultaneous CN NOESY | not available | not available | not available |
Software:
FELIX v2000 - spectral processing, peak peaking, peak assignments
NMRPipe - spectral processing
NMR spectrometers:
- Varian Unity 500 MHz
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts