BMRB Entry 5946
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5946
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Title: Complete Resonance assignments of a donor-strand complemented AfaD: the afimbrial invasin from Diffusely Adherent E. coli PubMed: 15213444
Deposition date: 2003-09-15 Original release date: 2004-07-06
Authors: Cota, Ernesto; Anderson, Kirstine; Peter, Simpson; Ho Ann, Chen; Bogdan, Nowicki; Josef, Kur
Citation: Cota, Ernesto; Chen, Ho An; Anderson, Kirstine; Simpson, Peter; du Merle, Laurence; Bernier-Febreau, Christine; Piatek, Rafa; Zalewska, Beata; Nowicki, Bogdan; Kur, Jozef; Le Bouguenec, Chantal; Matthews, Stephen. "Letter to the Editor: Complete resonance assignments of the "donor-strand complemented" AfaD: the afimbrial invasin from Diffusely Adherent E. coli " J. Biomol. NMR 29, 411-412 (2004).
Assembly members:
afaD-III, polymer, 153 residues, 16601.2 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
afaD-III: MRGSHHHHHHGSAELHLESR
GGSGTQLRDGAKVATGRIIC
REAHTGFHVWMNERQVDGRA
ERYVVQSKDGRHELRVRTGG
DGWSPVKGEGGKGVSRPGQE
EQVFFDVMADGNQDIAPGEY
RFSVGGACVVPQEDNKQGFT
PSGTTGTTKLTVT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 811 |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 133 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | afaD-dsc monomer | 1 |
Entities:
Entity 1, afaD-dsc monomer 153 residues - 16601.2 Da.
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | ALA | GLU | LEU | HIS | LEU | GLU | SER | ARG | ||||
| 3 | GLY | GLY | SER | GLY | THR | GLN | LEU | ARG | ASP | GLY | ||||
| 4 | ALA | LYS | VAL | ALA | THR | GLY | ARG | ILE | ILE | CYS | ||||
| 5 | ARG | GLU | ALA | HIS | THR | GLY | PHE | HIS | VAL | TRP | ||||
| 6 | MET | ASN | GLU | ARG | GLN | VAL | ASP | GLY | ARG | ALA | ||||
| 7 | GLU | ARG | TYR | VAL | VAL | GLN | SER | LYS | ASP | GLY | ||||
| 8 | ARG | HIS | GLU | LEU | ARG | VAL | ARG | THR | GLY | GLY | ||||
| 9 | ASP | GLY | TRP | SER | PRO | VAL | LYS | GLY | GLU | GLY | ||||
| 10 | GLY | LYS | GLY | VAL | SER | ARG | PRO | GLY | GLN | GLU | ||||
| 11 | GLU | GLN | VAL | PHE | PHE | ASP | VAL | MET | ALA | ASP | ||||
| 12 | GLY | ASN | GLN | ASP | ILE | ALA | PRO | GLY | GLU | TYR | ||||
| 13 | ARG | PHE | SER | VAL | GLY | GLY | ALA | CYS | VAL | VAL | ||||
| 14 | PRO | GLN | GLU | ASP | ASN | LYS | GLN | GLY | PHE | THR | ||||
| 15 | PRO | SER | GLY | THR | THR | GLY | THR | THR | LYS | LEU | ||||
| 16 | THR | VAL | THR |
Samples:
sample_1: afaD-III 0.8 mM
Ex-cond_1: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- . . . MHz
Related Database Links:
| PDB | |
| EMBL | CAA54118 CAA54119 CAA54120 CAW30800 CAW30817 |
| GB | AAG10405 AAG10406 AAG10513 AAK16478 AAK17182 |
| REF | WP_001014485 WP_001539577 WP_021545092 WP_021573025 WP_023909623 |
| SP | Q47038 |
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