BMRB Entry 5944
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5944
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Title: A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8 PubMed: 14500983
Deposition date: 2003-09-11 Original release date: 2003-12-19
Authors: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael
Citation: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael. "A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8" Science (Washington, DC, U. S.) 301, 1725-1728 (2003).
Assembly members:
CD4, polymer, 38 residues, Formula weight is not available
Lck, polymer, 29 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CD4: RCRHRRRQAERLSQIKRLLS
EKKTCQCPHRFQKTCSPI
Lck: SHPEDDWLENIDVCENCHYP
IVPLDGKGT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 195 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 359 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD4 cytoplasmic tail | 1 |
| 2 | Lck N-terminus | 2 |
| 3 | ZINC (II) ION | 3 |
Entities:
Entity 1, CD4 cytoplasmic tail 38 residues - Formula weight is not available
| 1 | ARG | CYS | ARG | HIS | ARG | ARG | ARG | GLN | ALA | GLU | ||||
| 2 | ARG | LEU | SER | GLN | ILE | LYS | ARG | LEU | LEU | SER | ||||
| 3 | GLU | LYS | LYS | THR | CYS | GLN | CYS | PRO | HIS | ARG | ||||
| 4 | PHE | GLN | LYS | THR | CYS | SER | PRO | ILE |
Entity 2, Lck N-terminus 29 residues - Formula weight is not available
| 1 | SER | HIS | PRO | GLU | ASP | ASP | TRP | LEU | GLU | ASN | ||||
| 2 | ILE | ASP | VAL | CYS | GLU | ASN | CYS | HIS | TYR | PRO | ||||
| 3 | ILE | VAL | PRO | LEU | ASP | GLY | LYS | GLY | THR |
Entity 3, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
Sample_1: CD4 cytoplasmic tail - Lck N terminus 0.5 mM; Lck 0.5 mM
Sample_2: CD4 cytoplasmic tail - Lck N terminus, [U-15N], 0.5 mM; Lck 0.5 mM
Sample_3: CD4 cytoplasmic tail - Lck N terminus, [U-15N; U-13C], 0.5 mM; Lck 0.5 mM
Condition_set_1: pH: 5.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | Condition_set_1 |
| HN(CO)CA | not available | not available | Condition_set_1 |
| HNCACB | not available | not available | Condition_set_1 |
| HN(CO)CACB | not available | not available | Condition_set_1 |
| CCONH | not available | not available | Condition_set_1 |
| HCCONH | not available | not available | Condition_set_1 |
| HCCH TOCSY | not available | not available | Condition_set_1 |
| 15N-NOESY | not available | not available | Condition_set_1 |
| 15N-TOCSY | not available | not available | Condition_set_1 |
| D2O NOESY | not available | not available | Condition_set_1 |
| D2O TOCSY | not available | not available | Condition_set_1 |
| Cd-H HMQC | not available | not available | Condition_set_1 |
| Cd-H NOESY-HMQC | not available | not available | Condition_set_1 |
Software:
FELIX - processing
DYANA - structure solution
X-PLOR - structure solution
NMR spectrometers:
- Bruker Varian 500 MHz
- Bruker Varian 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts