BMRB Entry 5929
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5929
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Title: NMR assignment of the hypothetical rhodanase domain At4g01050 from Arabidopsis thaliana PubMed: 15014235
Deposition date: 2003-09-01 Original release date: 2004-04-07
Authors: Pantoja-Uceda, David; Lopez-Mendez, Blanca; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter
Citation: Pantoja-Uceda, David; Lopez-Mendez, Blanca; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter. "Letter to the Editor: NMR assignment of the hypothetical rhodanese domain At4g01050 from Arabidopsis thaliana " J. Biomol. NMR 29, 207-208 (2004).
Assembly members:
single chain biopolymer, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
single chain biopolymer: GSSGSSGSAKNAYTKLGTDD
NAQLLDIRATADFRQVGSPN
IKGLGKKAVSTVYNGEDKPG
FLKKLSLKFKDPENTTLYIL
DKFDGNSELVAELVALNGFK
SAYAIKDGAEGPRGWLNSSL
PWIEPKKTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 908 |
| 13C chemical shifts | 559 |
| 15N chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rhodanase monomer | 1 |
Entities:
Entity 1, rhodanase monomer 134 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | ALA | LYS | ||||
| 2 | ASN | ALA | TYR | THR | LYS | LEU | GLY | THR | ASP | ASP | ||||
| 3 | ASN | ALA | GLN | LEU | LEU | ASP | ILE | ARG | ALA | THR | ||||
| 4 | ALA | ASP | PHE | ARG | GLN | VAL | GLY | SER | PRO | ASN | ||||
| 5 | ILE | LYS | GLY | LEU | GLY | LYS | LYS | ALA | VAL | SER | ||||
| 6 | THR | VAL | TYR | ASN | GLY | GLU | ASP | LYS | PRO | GLY | ||||
| 7 | PHE | LEU | LYS | LYS | LEU | SER | LEU | LYS | PHE | LYS | ||||
| 8 | ASP | PRO | GLU | ASN | THR | THR | LEU | TYR | ILE | LEU | ||||
| 9 | ASP | LYS | PHE | ASP | GLY | ASN | SER | GLU | LEU | VAL | ||||
| 10 | ALA | GLU | LEU | VAL | ALA | LEU | ASN | GLY | PHE | LYS | ||||
| 11 | SER | ALA | TYR | ALA | ILE | LYS | ASP | GLY | ALA | GLU | ||||
| 12 | GLY | PRO | ARG | GLY | TRP | LEU | ASN | SER | SER | LEU | ||||
| 13 | PRO | TRP | ILE | GLU | PRO | LYS | LYS | THR | SER | GLY | ||||
| 14 | PRO | SER | SER | GLY |
Samples:
sample_1: single chain biopolymer, [U-13C; U-15N], 1.10 mM; phosphate buffer 20 mM; NaCl 100 mM; dithiothreitol 1 mM; sodium azide 0.02 % (v/v); H2O 90 % (v/v); D2O 10 % (v/v)
condition_set_1: pH: 6.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| [1H,15N ]-HSQC | not available | not available | not available |
| [1H,13C]-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HA-coupled HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| (H)CC(CO)NH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| H(CCCO)NH | not available | not available | not available |
| 3D 15N-edited [1H,1H]-NOESY | not available | not available | not available |
| 13C-edited [1H,1H]-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AV 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE98457 |
| EMBL | CAB80914 |
| GB | AAB61039 AAL09804 AAL15331 AAM70579 AEE81973 |
| REF | NP_567209 XP_010424692 |
| SP | Q9M158 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts