BMRB Entry 5928
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5928
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Title: NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana PubMed: 15014234
Deposition date: 2003-09-01 Original release date: 2004-04-07
Authors: Lopez-Mendez, Blanca; Pantoja-Uceda, David; Tomizawa, Tadashi; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter
Citation: Lopez-Mendez, Blanca; Pantoja-Uceda, David; Tomizawa, Tadashi; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter. "Letter to the Editor: NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana" J. Biomol. NMR 29, 205-206 (2004).
Assembly members:
enth-vhs domain, polymer, 140 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
enth-vhs domain: GSSGSSGESYWRSRMIDAVT
SDEDKVAPVYKLEEICDLLR
SSHVSIVKEFSEFILKRLDN
KSPIVKQKALRLIKYAVGKS
GSEFRREMQRNSVAVRNLFH
YKGHPDPLKGDALNKAVRET
AHETISAIFSEENGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 585 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 924 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | enth-vhs monomer | 1 |
Entities:
Entity 1, enth-vhs monomer 140 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | SER | TYR | |
| 2 | TRP | ARG | SER | ARG | MET | ILE | ASP | ALA | VAL | THR | |
| 3 | SER | ASP | GLU | ASP | LYS | VAL | ALA | PRO | VAL | TYR | |
| 4 | LYS | LEU | GLU | GLU | ILE | CYS | ASP | LEU | LEU | ARG | |
| 5 | SER | SER | HIS | VAL | SER | ILE | VAL | LYS | GLU | PHE | |
| 6 | SER | GLU | PHE | ILE | LEU | LYS | ARG | LEU | ASP | ASN | |
| 7 | LYS | SER | PRO | ILE | VAL | LYS | GLN | LYS | ALA | LEU | |
| 8 | ARG | LEU | ILE | LYS | TYR | ALA | VAL | GLY | LYS | SER | |
| 9 | GLY | SER | GLU | PHE | ARG | ARG | GLU | MET | GLN | ARG | |
| 10 | ASN | SER | VAL | ALA | VAL | ARG | ASN | LEU | PHE | HIS | |
| 11 | TYR | LYS | GLY | HIS | PRO | ASP | PRO | LEU | LYS | GLY | |
| 12 | ASP | ALA | LEU | ASN | LYS | ALA | VAL | ARG | GLU | THR | |
| 13 | ALA | HIS | GLU | THR | ILE | SER | ALA | ILE | PHE | SER | |
| 14 | GLU | GLU | ASN | GLY | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: enth-vhs domain, [U-13C; U-15N], 1.20 mM; Tris buffer 20 mM; NaCl 100 mM; dithiothreitol 1 mM; sodium azide 0.02 % (v/v); H2O 90 % (v/v); D2O 10 % (v/v)
condition_set_1: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D [1H,15N ]-HSQC | not available | not available | not available |
| 2D [1H,13C]-HSQC | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CA)CO | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HBHA(CO)NH | not available | not available | not available |
| 3D (H)CC(CO)NH | not available | not available | not available |
| 3D HCCH-COSY | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D H(CCCO)NH | not available | not available | not available |
| 3D (H)CCH-TOCSY | not available | not available | not available |
| 3D 15N-edited [1H,1H]-NOESY | not available | not available | not available |
| 3D 13C-edited [1H,1H]-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AV 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB01267 BAH57080 |
| GB | AAB63649 AAK25955 AAK64101 AEE75792 |
| REF | NP_566540 XP_010419285 XP_010465644 XP_010465645 XP_010487480 |
| SP | Q9C5H4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts