BMRB Entry 5908
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5908
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Title: Backbone 1HN and 15N Chemical Shift Assignments for Sac7d V30I
Deposition date: 2003-08-19 Original release date: 2003-10-06
Authors: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John
Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d" J. Mol. Biol. ., .-..
Assembly members:
Sac7d, polymer, 66 residues, 7623 Da.
Natural source: Common Name: Sulfolobus acidocaldarius Brock et al. Taxonomy ID: 2285 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus acidocaldarius
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sac7d: MVKVKFKYKGEEKEVDTSKI
KKVWRVGKMISFTYDDNGKT
GRGAVSEKDAPKELLDMLAR
AEREKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 67 |
| 15N chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sac7d V30I subunit 1 | 1 |
Entities:
Entity 1, Sac7d V30I subunit 1 66 residues - 7623 Da.
| 1 | MET | VAL | LYS | VAL | LYS | PHE | LYS | TYR | LYS | GLY | ||||
| 2 | GLU | GLU | LYS | GLU | VAL | ASP | THR | SER | LYS | ILE | ||||
| 3 | LYS | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | ILE | ||||
| 4 | SER | PHE | THR | TYR | ASP | ASP | ASN | GLY | LYS | THR | ||||
| 5 | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ALA | ||||
| 6 | PRO | LYS | GLU | LEU | LEU | ASP | MET | LEU | ALA | ARG | ||||
| 7 | ALA | GLU | ARG | GLU | LYS | LYS |
Samples:
Sac7d_V30I: Sac7d, [U-98% 15N], 1.18 mM; KCl 0.3 M; H2O 90%; D2O 10%; DSS 100 uM
sample_conds: pH: 4.06; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N HSQC | Sac7d_V30I | not available | sample_conds |
| 3D HSQC-TOCSY | Sac7d_V30I | not available | sample_conds |
| 3D HSQC-NOESY | Sac7d_V30I | not available | sample_conds |
Software:
NMRPipe - data processing, transformation, referencing
NMRView v5 - sequential assignments
NMR spectrometers:
- Varian VXR-S 500 MHz
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 5905 |
| PDB | |
| GB | AAA80315 AAY79492 AGE70041 AGE72316 AHC50588 |
| REF | WP_011276993 YP_007433189 YP_007435464 YP_254785 |
| SP | P13123 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts