BMRB Entry 5907
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5907
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Title: Assignment of 1H, 13C and 15N Resonances of the Human Ku80 C-terminal Domain PubMed: 15016365
Deposition date: 2003-08-19 Original release date: 2004-08-30
Authors: Zhang, Ziming; Hu, Weidong; Chen, Yuan
Citation: Zhang, Ziming; Hu, Weidong; Cano, L.; Lee, T.; Chen, D.; Chen, Yuan. "Solution structure of the C-terminal domain of Ku80 suggests important sites for protein-protein interactions." Structure (Camb) 12, 495-502 (2004).
Assembly members:
Human Ku80 C-terminal Domain, polymer, 152 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Human Ku80 C-terminal Domain: MHHHHHHKLKTEQGGAHFSV
SSLAEGSVTSVGSVNPAENF
RVLVKQKKASFEEASNQLIN
HIEQFLDTNETPYFMKSIDC
IRAFREEAIKFSEEQRFNNF
LKALQEKVEIKQLNHFWEIV
VQDGITLITKEEASGSSVTA
EEAKKFLAPKDK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 601 |
| 15N chemical shifts | 155 |
| 1H chemical shifts | 977 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ku80 CTD | 1 |
Entities:
Entity 1, Ku80 CTD 152 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | LYS | LEU | LYS | ||||
| 2 | THR | GLU | GLN | GLY | GLY | ALA | HIS | PHE | SER | VAL | ||||
| 3 | SER | SER | LEU | ALA | GLU | GLY | SER | VAL | THR | SER | ||||
| 4 | VAL | GLY | SER | VAL | ASN | PRO | ALA | GLU | ASN | PHE | ||||
| 5 | ARG | VAL | LEU | VAL | LYS | GLN | LYS | LYS | ALA | SER | ||||
| 6 | PHE | GLU | GLU | ALA | SER | ASN | GLN | LEU | ILE | ASN | ||||
| 7 | HIS | ILE | GLU | GLN | PHE | LEU | ASP | THR | ASN | GLU | ||||
| 8 | THR | PRO | TYR | PHE | MET | LYS | SER | ILE | ASP | CYS | ||||
| 9 | ILE | ARG | ALA | PHE | ARG | GLU | GLU | ALA | ILE | LYS | ||||
| 10 | PHE | SER | GLU | GLU | GLN | ARG | PHE | ASN | ASN | PHE | ||||
| 11 | LEU | LYS | ALA | LEU | GLN | GLU | LYS | VAL | GLU | ILE | ||||
| 12 | LYS | GLN | LEU | ASN | HIS | PHE | TRP | GLU | ILE | VAL | ||||
| 13 | VAL | GLN | ASP | GLY | ILE | THR | LEU | ILE | THR | LYS | ||||
| 14 | GLU | GLU | ALA | SER | GLY | SER | SER | VAL | THR | ALA | ||||
| 15 | GLU | GLU | ALA | LYS | LYS | PHE | LEU | ALA | PRO | LYS | ||||
| 16 | ASP | LYS |
Samples:
Sample_1: Human Ku80 C-terminal Domain1.0 – 1.5 mM
Experiment_conditions: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | Sample_1 | not available | Experiment_conditions |
| HN(CA)CO | Sample_1 | not available | Experiment_conditions |
| CBCA(CO)NH | Sample_1 | not available | Experiment_conditions |
| HNCACB | Sample_1 | not available | Experiment_conditions |
| C(CO)NH | Sample_1 | not available | Experiment_conditions |
| HC(CO)NH | Sample_1 | not available | Experiment_conditions |
| TOCSY | Sample_1 | not available | Experiment_conditions |
| HCCHTOCSY | Sample_1 | not available | Experiment_conditions |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 5912 |
| PDB | |
| DBJ | BAD96323 BAE01114 BAF83429 BAH24115 |
| EMBL | CAA40736 CAH92321 |
| GB | AAA36154 AAA59475 AAH19027 AAH95442 AAR11279 |
| REF | NP_001126362 NP_001244295 NP_066964 XP_001151873 XP_003254095 |
| SP | P13010 |
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