BMRB Entry 5874
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5874
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Title: Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97 PubMed: 14752269
Deposition date: 2003-07-18 Original release date: 2003-12-05
Authors: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Shaw, Anthony; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen
Citation: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Shaw, Anthony; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen. "Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97" J. Biomol. NMR 28, 309-310 (2004).
Assembly members:
p47, polymer, 174 residues, 41000 Da.
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
p47: MAEERQDALREFVAVTGAEE
DRARFFLESAGWDLQIALAS
FYEDGGDEDIVTISQATPSS
VSRGTAPSDNRVTSFRDLIH
DQDEEEEEEEGQRFYAGGSE
RSGQQIVGPPRKKSPNELVD
DLFKGAKEHGAVAVERVTKS
PGETSKPRPFAGGGYRLGAA
PEEESAYVAGERRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 282 |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 759 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p47 residues 1-174, p47 monomer | 1 |
Entities:
Entity 1, p47 residues 1-174, p47 monomer 174 residues - 41000 Da.
| 1 | MET | ALA | GLU | GLU | ARG | GLN | ASP | ALA | LEU | ARG | ||||
| 2 | GLU | PHE | VAL | ALA | VAL | THR | GLY | ALA | GLU | GLU | ||||
| 3 | ASP | ARG | ALA | ARG | PHE | PHE | LEU | GLU | SER | ALA | ||||
| 4 | GLY | TRP | ASP | LEU | GLN | ILE | ALA | LEU | ALA | SER | ||||
| 5 | PHE | TYR | GLU | ASP | GLY | GLY | ASP | GLU | ASP | ILE | ||||
| 6 | VAL | THR | ILE | SER | GLN | ALA | THR | PRO | SER | SER | ||||
| 7 | VAL | SER | ARG | GLY | THR | ALA | PRO | SER | ASP | ASN | ||||
| 8 | ARG | VAL | THR | SER | PHE | ARG | ASP | LEU | ILE | HIS | ||||
| 9 | ASP | GLN | ASP | GLU | GLU | GLU | GLU | GLU | GLU | GLU | ||||
| 10 | GLY | GLN | ARG | PHE | TYR | ALA | GLY | GLY | SER | GLU | ||||
| 11 | ARG | SER | GLY | GLN | GLN | ILE | VAL | GLY | PRO | PRO | ||||
| 12 | ARG | LYS | LYS | SER | PRO | ASN | GLU | LEU | VAL | ASP | ||||
| 13 | ASP | LEU | PHE | LYS | GLY | ALA | LYS | GLU | HIS | GLY | ||||
| 14 | ALA | VAL | ALA | VAL | GLU | ARG | VAL | THR | LYS | SER | ||||
| 15 | PRO | GLY | GLU | THR | SER | LYS | PRO | ARG | PRO | PHE | ||||
| 16 | ALA | GLY | GLY | GLY | TYR | ARG | LEU | GLY | ALA | ALA | ||||
| 17 | PRO | GLU | GLU | GLU | SER | ALA | TYR | VAL | ALA | GLY | ||||
| 18 | GLU | ARG | ARG | ARG |
Samples:
sample_1: p47, [U-13C; U-15N], 0.5 mM
ex-cond: pH: 5.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
Software:
NMRPipe -
NMRView -
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian . 800 MHz
Related Database Links:
| DBJ | BAA21659 BAA91731 BAB28604 BAC30250 BAD97061 |
| EMBL | CAA71742 CAH90897 |
| GB | AAD44488 AAH02801 AAH50936 AAH72464 AAI02957 |
| REF | NP_001029729 NP_001125510 NP_001231372 NP_001278003 NP_057227 |
| SP | O35987 Q3SZC4 Q5RBG3 Q9CZ44 Q9UNZ2 |
| TPG | DAA23261 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts