BMRB Entry 5696

Name: 1H, 15N and 13C resonance assignments of the PDZ domain of ZASP
Published: 2004-04-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5696

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 15N and 13C resonance assignments of the PDZ domain of ZASP PubMed: 15062084

Deposition date: 2003-02-17 Original release date: 2004-04-07

Authors: Au, Yunghan; Atkinson, R.; Faulkner, Georgine; Frenkiel, Thomas; Joseph, Catherine; Kelly, Geoff; Muskett, Frederick; Pallavicini, Alberto; Pastore, Annalisa

Citation: Au, Yunghan; Atkinson, R.; Guerrini, R.; Kelly, Geoff; Joseph, Catherine; Martin, S.; Muskett, Frederick; Pallavicini, Alberto; Faulkner, Georgine; Pastore, Annalisa. "Solution Structure of ZASP PDZ Domain; Implications for Sarcomere Ultrastructure and Enigma Family Redundancy" Structure (Cambridge, MA, U. S.) 12, 611-622 (2004).

Assembly members:
Z-band alternatively spliced PDZ motif protein, polymer, 85 residues, 9139.40 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
Z-band alternatively spliced PDZ motif protein: MAYSVTLTGPGPWGFRLQGG KDFNMPLTISRITPGSKAAQ SQLSQGDLVVAIDGVNTDTM THLEAQNKIKSASYNLSLTL QKSKR

Data sets:

assigned_chemical_shifts
- Assignment_1

Data type	Count
1H chemical shifts	577
13C chemical shifts	335
15N chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZASP-PDZ Domain	1

Entities:

Entity 1, ZASP-PDZ Domain 85 residues - 9139.40 Da.

1

MET

ALA

TYR

SER

VAL

THR

LEU

THR

GLY

PRO

2

GLY

PRO

TRP

GLY

PHE

ARG

LEU

GLN

GLY

3

LYS

ASP

PHE

ASN

MET

PRO

LEU

THR

ILE

SER

4

ARG

ILE

THR

PRO

GLY

SER

LYS

ALA

GLN

5

SER

GLN

LEU

SER

GLN

GLY

ASP

LEU

VAL

6

ALA

ILE

ASP

GLY

VAL

ASN

THR

ASP

THR

MET

7

THR

HIS

LEU

GLU

ALA

GLN

ASN

LYS

ILE

LYS

8

SER

ALA

SER

TYR

ASN

LEU

SER

LEU

THR

LEU

9

GLN

LYS

SER

LYS

ARG

Samples:

Sample_1: Z-band alternatively spliced PDZ motif protein, [U-15N; U-13C], 0.5 – 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % v/v; deuterium oxide 10 % v/v

Sample_2: Z-band alternatively spliced PDZ motif protein, [U-15N], 0.5 – 1.5 mM

Condition_1: pH: 6.6; temperature: 300 K; ionic strength: 0.02 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCA	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
CBCANH	not available	not available	not available
HNCO	not available	not available	not available
HCCH TOCSY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available
1H-13C NOESY	not available	not available	not available
HNHA	not available	not available	not available

Software:

nmrPipe - processing

XEASY - analysis

NMR spectrometers:

Varian UnityPlus 500 MHz
Varian UnityPlus 600 MHz
Varian UnityPlus 800 MHz

BMRB	10061
PDB	1RGW 1WJL
DBJ	BAA31588 BAB23128 BAD32258 BAE23262 BAE25016
EMBL	CAB46727 CAB46728 CAB46729 CAB46747
GB	AAD42950 AAD42951 AAF33847 AAF33848 AAH10929
REF	NP_001030493 NP_001034160 NP_001034161 NP_001034162 NP_001034163
SP	O75112 Q9JKS4
TPG	DAA14160

Biological Magnetic Resonance Data Bank