BMRB Entry 5669
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5669
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Title: Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4 PubMed: 12644454
Deposition date: 2003-01-23 Original release date: 2003-06-10
Authors: Wang, B.; Alam, S.; Meyer, H.; Payne, M.; Stemmler, T.; Davis, D.; Sundquist, W.
Citation: Wang, B.; Alam, S.; Meyer, H.; Payne, M.; Stemmler, T.; Davis, D.; Sundquist, W.. "Structure and Ubiquitin Interactions of the Conserved Zinc Finger Domain of Npl4" J. Biol. Chem. 278, 20225-20234 (2003).
Assembly members:
Npl4 Zinc Finger, polymer, 31 residues, 3373 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Npl4 Zinc Finger: GSTSAMWACQHCTFMNQPGT
GHCEMCSLPRT
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 109 |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 164 |
| coupling constants | 13 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Npl4 Zinc Finger domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Npl4 Zinc Finger domain 31 residues - 3373 Da.
| 1 | GLY | SER | THR | SER | ALA | MET | TRP | ALA | CYS | GLN | ||||
| 2 | HIS | CYS | THR | PHE | MET | ASN | GLN | PRO | GLY | THR | ||||
| 3 | GLY | HIS | CYS | GLU | MET | CYS | SER | LEU | PRO | ARG | ||||
| 4 | THR |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Npl4 Zinc Finger, [U-15N; U-13C], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; BME 5 mM; H2O 90%; D2O 10%
sample_2: Npl4 Zinc Finger, [U-95% 15N], 1 mM
sample_cond_1: ionic strength: 75 mM; pH: 5.5; pressure: 1 atm; temperature: 291 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | not available | not available | sample_cond_1 |
| HN(CA)CO | not available | not available | sample_cond_1 |
| H(CCO)NH-TOCSY | not available | not available | sample_cond_1 |
| (H)C(CO)NH-TOCSY | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
Software:
CNS v1.1 - refinement
DYANA v1.5 - refinement
FELIX v97 - processing
SPARKY v3.106 - data analysis
NMR spectrometers:
- Varian UNITYplus 500 MHz
- Varian Inova 600 MHz
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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