BMRB Entry 5556
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5556
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Title: PHD Fingers as Protein Scaffolds PubMed: 12842043
Deposition date: 2002-10-10 Original release date: 2003-08-01
Authors: Kwan, A.; Matthews, J.; Mackay, J.
Citation: Kwan, A.; Gell, D.; Verger, A.; Crossley, M.; Matthews, J.; Mackay, J.. "Engineering a Protein Scaffold from a PHD Finger" Structure 11, 803-813 (2003).
Assembly members:
PHD, polymer, 61 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PHD: GPLGSDHHMEFCRVCKDGGE
LLCCDTCPSSYHIHCLRPAL
YEVPDGEWQCPRCTCPALKG
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 311 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mi2-beta with WSTF | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Mi2-beta with WSTF 61 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ASP | HIS | HIS | MET | GLU | ||||
| 2 | PHE | CYS | ARG | VAL | CYS | LYS | ASP | GLY | GLY | GLU | ||||
| 3 | LEU | LEU | CYS | CYS | ASP | THR | CYS | PRO | SER | SER | ||||
| 4 | TYR | HIS | ILE | HIS | CYS | LEU | ARG | PRO | ALA | LEU | ||||
| 5 | TYR | GLU | VAL | PRO | ASP | GLY | GLU | TRP | GLN | CYS | ||||
| 6 | PRO | ARG | CYS | THR | CYS | PRO | ALA | LEU | LYS | GLY | ||||
| 7 | LYS |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHD 1 mM; DTT 1 mM; sodium phosphate buffer 10 mM; NaCl 150 mM; H2O 95%; D2O 5%
sample_cond_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
Software:
xwinnmr v2.5 - processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - refinement
ARIA v1.1.2 - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |