BMRB Entry 5394

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PDB ID: 1lpw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5394
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Sculpting of the Eukaryotic Branch Site Recognition Motif by a Conserved Pseudouridine PubMed: 12426583

Deposition date: 2002-06-12 Original release date: 2005-11-14

Authors: Newby, Meredith; Greenbaum, Nancy

Citation: Newby, Meredith; Greenbaum, Nancy. "Sculpting of the Spliceosomal Branch Site Recognition Motif by a Conserved Pseudouridine" Nat. Struct. Biol. 9, 958-965 (2002).

Assembly members:
U2 snRNA - intron duplex, polymer, 9 residues, Formula weight is not available
U2 snRNA - intron duplex, polymer, 10 residues, Formula weight is not available

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
U2 snRNA - intron duplex: GGUGXAGUA
U2 snRNA - intron duplex: UACUAACACC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- chemical_shift_set_2

Data type	Count
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U2 snRNA strand	1
2	Intron strand	2

Entities:

Entity 1, U2 snRNA strand 9 residues - Formula weight is not available

1

G

U

G

PSU

A

G

U

A

Entity 2, Intron strand 10 residues - Formula weight is not available

1

U

A

C

U

A

C

A

C

Samples:

sample_1: U2 snRNA - intron duplex 1.0 mM; U2 snRNA - intron duplex 1.0 mM; sodium phosphate 10 mM; D2O 10%; H2O 90%

sample_2: U2 snRNA - intron duplex 1.0 mM; U2 snRNA - intron duplex 1.0 mM; sodium phosphate 10 mM; D2O 100%

sample_cond_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 278 K

sample_cond_2: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available

Software:

VNMR v5.3 - collection

SPARKY v3.69 - data analysis

X-PLOR v3.851 - refinement, structure Solution

NMR spectrometers:

Varian UNITYplus 720 MHz