BMRB Entry 5364
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5364
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Title: Structure of the C-terminal FG-binding domain of human Tap PubMed: 11875519
Deposition date: 2002-05-07 Original release date: 2002-05-30
Authors: Grant, Richard; Neuhaus, David; Stewart, Murray
Citation: Grant, Richard; Hurt, Ed; Neuhaus, David; Stewart, Murray. "Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1" Nat. Struct. Biol. 9, 247-251 (2002).
Assembly members:
Tap C-terminal domain, polymer, 69 residues, 7712.6 Da.
Natural source: Common Name: HUMAN Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Tap C-terminal domain: PAPTPSSSPVPTLSPEQQEM
LQAFSTQSGMNLEWSQKCLQ
DNNWDYTRSAQAFTHLKAKG
EIPEVAFMK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 456 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tap | 1 |
Entities:
Entity 1, Tap 69 residues - 7712.6 Da.
| 1 | PRO | ALA | PRO | THR | PRO | SER | SER | SER | PRO | VAL | ||||
| 2 | PRO | THR | LEU | SER | PRO | GLU | GLN | GLN | GLU | MET | ||||
| 3 | LEU | GLN | ALA | PHE | SER | THR | GLN | SER | GLY | MET | ||||
| 4 | ASN | LEU | GLU | TRP | SER | GLN | LYS | CYS | LEU | GLN | ||||
| 5 | ASP | ASN | ASN | TRP | ASP | TYR | THR | ARG | SER | ALA | ||||
| 6 | GLN | ALA | PHE | THR | HIS | LEU | LYS | ALA | LYS | GLY | ||||
| 7 | GLU | ILE | PRO | GLU | VAL | ALA | PHE | MET | LYS |
Samples:
sample_1: Tap C-terminal domain 3 mM; potassium phosphate 25 mM; sodium chloride 50 mM; deuterium oxide 10%; sodium trimethylsilyl propionate 0.1 mM
sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 0.07 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| DQ-CORR | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DMX 600 MHz
- Bruker Avance 800 MHz