BMRB Entry 5349
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5349
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Title: PBX Homeodomain-DNA complex
Deposition date: 2002-04-19 Original release date: 2003-01-06
Authors: Sprules, Tara; Green, Nancy; Featherstone, Mark; Gehring, Kalle
Citation: Sprules, Tara; Green, Nancy; Featherstone, Mark; Gehring, Kalle. "Lock and Key Binding of the HOX YPWM Peptide to the PBX Homeodomain" J. Biol. Chem. 278, 1053-1058 (2003).
Assembly members:
PBX homeodomain, polymer, 82 residues, Formula weight is not available
DNA, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PBX homeodomain: MARRKRRNFNKQATEILNEY
FYSHLSNPYPSEEAKEELAK
KSGITVSQVSNWFGNKRIRY
KKNIGKFQEEANIYAAKTAV
TA
DNA: GCGCATGATTGCCCGGGCAA
TCATGCGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 1H chemical shifts | 778 |
| 15N chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Extended PBX Homeodomain | 1 |
| 2 | 14mer DNA duplex | 2 |
Entities:
Entity 1, Extended PBX Homeodomain 82 residues - Formula weight is not available
| 1 | MET | ALA | ARG | ARG | LYS | ARG | ARG | ASN | PHE | ASN | ||||
| 2 | LYS | GLN | ALA | THR | GLU | ILE | LEU | ASN | GLU | TYR | ||||
| 3 | PHE | TYR | SER | HIS | LEU | SER | ASN | PRO | TYR | PRO | ||||
| 4 | SER | GLU | GLU | ALA | LYS | GLU | GLU | LEU | ALA | LYS | ||||
| 5 | LYS | SER | GLY | ILE | THR | VAL | SER | GLN | VAL | SER | ||||
| 6 | ASN | TRP | PHE | GLY | ASN | LYS | ARG | ILE | ARG | TYR | ||||
| 7 | LYS | LYS | ASN | ILE | GLY | LYS | PHE | GLN | GLU | GLU | ||||
| 8 | ALA | ASN | ILE | TYR | ALA | ALA | LYS | THR | ALA | VAL | ||||
| 9 | THR | ALA |
Entity 2, 14mer DNA duplex 28 residues - Formula weight is not available
| 1 | DG | DC | DG | DC | DA | DT | DG | DA | DT | DT | ||||
| 2 | DG | DC | DC | DC | DG | DG | DG | DC | DA | DA | ||||
| 3 | DT | DC | DA | DT | DG | DC | DG | DC |
Samples:
sample_1: PBX homeodomain, [U-15N], 1.5 mM; DNA 1.5 mM; DSS 0.2 mM; sodium phosphate 10 mM
sample_2: PBX homeodomain, [U-15N; U-13C], 1.9 mM; DNA 1.9 mM; DSS 0.2 mM; sodium phosphate 10 mM
sample_3: PBX homeodomain 1.4 mM; DNA 1.4 mM; DSS 0.2 mM; sodium phosphate 10 mM
standard_conditions: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC-NOESY | not available | not available | standard_conditions |
| 13C HSQC-NOESY | not available | not available | standard_conditions |
| HNHA | not available | not available | standard_conditions |
| 2D NOESY | not available | not available | standard_conditions |
| CBCACONH | not available | not available | standard_conditions |
| 13C HSQC | not available | not available | standard_conditions |
| 13C-filtered NOESY | not available | not available | standard_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 4357 4358 4359 |
| PDB | |
| DBJ | BAA96135 BAA96136 BAB83538 BAF84104 BAG10741 |
| GB | AAA21832 AAA36484 AAA36764 AAA60031 AAB71191 |
| PIR | B33061 |
| REF | NP_001094151 NP_001128334 NP_001179697 NP_001191890 NP_001191892 |
| SP | P40424 P41778 |
| TPG | DAA32038 |
Download simulated HSQC data in one of the following formats:
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