BMRB Entry 5335
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5335
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Title: Backbone and side chain 1H, 13C, and 15N chemical shift assignments for E.coli protein YacG PubMed: 12211008
Deposition date: 2002-03-27 Original release date: 2002-09-09
Authors: Ramelot, Theresa; Kennedy, Michael
Citation: Ramelot, Theresa; Cort, J.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "NMR Structure of the Eschericia coli Protein YacG: A Novel Sequence Motif in the Zinc-finger Family of Proteins " Proteins 49, 289-293 (2002).
Assembly members:
YacG, polymer, 68 residues, 7306 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Eschericia coli
Experimental source: Production method: recombinant technology Host organism: Eschericia coli
Entity Sequences (FASTA):
YacG: GSHMSETITVNCPTCGKTVV
WGEISPFRPFCSKRCQLIDL
GEWAAEEKRIPSSGDLSESD
DWSEEPKQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 1H chemical shifts | 385 |
| 15N chemical shifts | 61 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YacG | 1 |
| 2 | zinc ion | 2 |
Entities:
Entity 1, YacG 68 residues - 7306 Da.
| 1 | GLY | SER | HIS | MET | SER | GLU | THR | ILE | THR | VAL | ||||
| 2 | ASN | CYS | PRO | THR | CYS | GLY | LYS | THR | VAL | VAL | ||||
| 3 | TRP | GLY | GLU | ILE | SER | PRO | PHE | ARG | PRO | PHE | ||||
| 4 | CYS | SER | LYS | ARG | CYS | GLN | LEU | ILE | ASP | LEU | ||||
| 5 | GLY | GLU | TRP | ALA | ALA | GLU | GLU | LYS | ARG | ILE | ||||
| 6 | PRO | SER | SER | GLY | ASP | LEU | SER | GLU | SER | ASP | ||||
| 7 | ASP | TRP | SER | GLU | GLU | PRO | LYS | GLN |
Entity 2, zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: YacG, [U-13C; U-15N], 1 mM; phosphate buffer 25 mM; NaCl 450 mM
sample_2: YacG, [U-15N], 1 – 3 mM; phosphate buffer 25 mM; NaCl 450 mM
cond_1: pH: 6.5 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | cond_1 |
| 1H-13C HSQC (aliph) | not available | not available | cond_1 |
| 15N-edited NOESY-HQSC | not available | not available | cond_1 |
| HCC-TOCSY-NNH | not available | not available | cond_1 |
| HCCH-TOCSY | not available | not available | cond_1 |
| CN-NOESY-HSQC | not available | not available | cond_1 |
| HNCO | not available | not available | cond_1 |
| HNCACB | not available | not available | cond_1 |
| CBCA(CO)NH | not available | not available | cond_1 |
| CCC-TOCSY-NNH | not available | not available | cond_1 |
| HNHA | not available | not available | cond_1 |
| 4D CC-NOESY | not available | not available | cond_1 |
| 1H-13C HSQC (alpha) | not available | not available | cond_1 |
| 1H-13C HSQC (arom) | not available | not available | cond_1 |
Software:
Felix v98 - processing data
XPLOR v3.84 - structure calculations
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
- Varian Inova 800 MHz
Related Database Links:
| SWISS-PROT | P0A8H8 P0A8H9 A7ZW52 B1IR78 A7ZHJ2 |
| REF | NP_706056 NP_308132 NP_414643 AP_000763 NP_285797 |
| GenBank | AAN78619 AAP15644 AAG54405 AAN41763 AAC73212 |
| DBJ | BAB96668 BAB33528 |
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts