BMRB Entry 5178
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5178
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Title: Solution Structure of the Fibronectin type III Domain from Bacillus circulans WL-12 Chitinase A1 PubMed: 11600504
Deposition date: 2001-10-15 Original release date: 2001-11-20
Authors: Jee, JunGoo; Ikegami, Takahisa; Hashimoto, Masayuki; Kawabata, Takeshi; Ikeguchi, Mitsunori; Watanabe, Takeshi; Shirakawa, Masahiro
Citation: Jee, JunGoo; Ikegami, Takahisa; Hashimoto, Masayuki; Kawabata, Takeshi; Ikeguchi, Mitsunori; Watanabe, Takeshi; Shirakawa, Masahiro. "Solution Structure of the Fibronectin type III Domain from Bacillus circulans WL-12 Chitinase A1" J. Biol. Chem. 277, 1388-1397 (2002).
Assembly members:
chitinase FnIII domain, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: soil bacteria Taxonomy ID: 1397 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus circulans
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
chitinase FnIII domain: HMAPTAPTNLASTAQTTSSI
TLSWTASTDNVGVTGYDVYN
GTALATTVTGTTATISGLAA
DTSYTFTVKAKDAAGNVSAA
SNAVSVKT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 316 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 505 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FnIIID domain 2 | 1 |
Entities:
Entity 1, FnIIID domain 2 88 residues - Formula weight is not available
| 1 | HIS | MET | ALA | PRO | THR | ALA | PRO | THR | ASN | LEU | ||||
| 2 | ALA | SER | THR | ALA | GLN | THR | THR | SER | SER | ILE | ||||
| 3 | THR | LEU | SER | TRP | THR | ALA | SER | THR | ASP | ASN | ||||
| 4 | VAL | GLY | VAL | THR | GLY | TYR | ASP | VAL | TYR | ASN | ||||
| 5 | GLY | THR | ALA | LEU | ALA | THR | THR | VAL | THR | GLY | ||||
| 6 | THR | THR | ALA | THR | ILE | SER | GLY | LEU | ALA | ALA | ||||
| 7 | ASP | THR | SER | TYR | THR | PHE | THR | VAL | LYS | ALA | ||||
| 8 | LYS | ASP | ALA | ALA | GLY | ASN | VAL | SER | ALA | ALA | ||||
| 9 | SER | ASN | ALA | VAL | SER | VAL | LYS | THR |
Samples:
sample_1: chitinase FnIII domain, [U-15N; U-13C], 1.0 mM; phosphate buffer 20 mM; KCl 50 mM
ex-cond_1: pH: 6.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DRX 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB |
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