BMRB Entry 5022

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5022

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Converting a DNA Damage Checkpoint Effector (UmuD2C) into a Lesion Bypass Polymerase (UmuD'2C) PubMed: 11483531

Deposition date: 2001-05-21 Original release date: 2003-01-07

Authors: Ferentz, A.; Walker, G.; Wagner, G.

Citation: Ferentz, A.; Walker, G.; Wagner, G.. "Converting a DNA Damage Checkpoint Effector (UmuD2C) into a Lesion Bypass Polymerase (UmuD'2C) " EMBO J. 20, 4287-4298 (2001).

Assembly members:
UMUD PROTEIN, polymer, 115 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
UMUD PROTEIN: AFPSPAADYVEQRIDLNQLL IQHPSATYFVKASGDSMIDG GISDGDLLIVDSAITASHGD IVIAAVDGEFTVKKLQLRPT VQLIPMNSAYSPITISSEDT LDVFGVVIHVVKAMR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	583
13C chemical shifts	290
15N chemical shifts	106

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UMUD PROTEIN, chain A	1
2	UMUD PROTEIN, chain B	1

Entities:

Entity 1, UMUD PROTEIN, chain A 115 residues - Formula weight is not available

1

ALA

PHE

PRO

SER

PRO

ALA

ASP

TYR

VAL

2

GLU

GLN

ARG

ILE

ASP

LEU

ASN

GLN

LEU

3

ILE

GLN

HIS

PRO

SER

ALA

THR

TYR

PHE

VAL

4

LYS

ALA

SER

GLY

ASP

SER

MET

ILE

ASP

GLY

5

GLY

ILE

SER

ASP

GLY

ASP

LEU

ILE

VAL

6

ASP

SER

ALA

ILE

THR

ALA

SER

HIS

GLY

ASP

7

ILE

VAL

ILE

ALA

VAL

ASP

GLY

GLU

PHE

8

THR

VAL

LYS

LEU

GLN

LEU

ARG

PRO

THR

9

VAL

GLN

LEU

ILE

PRO

MET

ASN

SER

ALA

TYR

10

SER

PRO

ILE

THR

ILE

SER

GLU

ASP

THR

11

LEU

ASP

VAL

PHE

GLY

VAL

ILE

HIS

VAL

12

VAL

LYS

ALA

MET

ARG

Samples:

sample_1: UMUD PROTEIN, [U-15N], 0.9 mM; NaCl 150 mM; phosphate 10 mM; DTT 1 mM; EDTA 0.1 mM; H2O 95%; D2O 5%

sample_2: UMUD PROTEIN, [U-15N; U-13C], 1.3 mM; NaCl 150 mM; phosphate 20 mM; DTT 1 mM; EDTA 0.1 mM; H2O 95%; D2O 5%

sample_3: UMUD PROTEIN 1.5 mM; NaCl 150 mM; phosphate 10 mM; DTT 1 mM; EDTA 0.1 mM; H2O 95%; D2O 5%

sample_4: UMUD PROTEIN 1.4 mM; NaCl 150 mM; phosphate 10 mM; DTT 1 mM; EDTA 0.1 mM; D2O 100%

sample_cond_1: pH: 6.0; temperature: 303 K; ionic strength: 160 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
HNHA	not available	not available	not available
2D NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available

Software:

FELIX v97.0 - processing

XEASY v1.3.9 - data analysis

X-PLOR v3.851 - refinement

NMR spectrometers:

Varian VXR 500 MHz

PDB	1AY9 1I4V 1UMU
DBJ	BAA36030 BAB35101 BAG76755 BAI24995 BAI30119
EMBL	CAP75718 CAQ31685 CAQ98062 CAR02572 CAR07525
GB	AAA24728 AAA98073 AAC74267 AAG56034 AAN42787
REF	NP_287422 NP_309705 NP_415701 NP_707080 NP_753536
SP	P0AG11 P0AG12 P0AG13

Biological Magnetic Resonance Data Bank