BMRB Entry 4927
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4927
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Title: 1H, 13C, 15N Resonance Assignments of a Circular Permuted Variant of CV-N (cpCV-N)
Deposition date: 2000-12-18 Original release date: 2002-04-04
Authors: Barrientos, Laura; Campos-Olivas, Ramon; Gronenborn, Angela
Citation: Barrientos, Laura; Campos-Olivas, Ramon; Louis, John; Fiser, Andras; Sali, Andrej; Gronenborn, Angela. "Letter to the editor: 1H, 13C, 15N Resonance Assignments and Fold Verification of a Circular Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N" J. Biomol. NMR 19, 289-290 (2001).
Assembly members:
Cyanovirin-N, polymer, 102 residues, 11013 Da.
Natural source: Common Name: Cyanobacterium Taxonomy ID: not available Superkingdom: Eubacteria Kingdom: not available Genus/species: Nostoc ellipsosporum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Cyanovirin-N: GLGKFIETCRNTQLAGSSEL
AAECKTRAQQFVSTKINLDD
HIANIDGTLKWQPSNFSQTC
YNSAIQGSVLTSTCERTNGG
YNTSSIDLNSVIENVDGSLK
YE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 632 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Circular Permuted Cyanovirin-N, cpCV-N monomer | 1 |
Entities:
Entity 1, Circular Permuted Cyanovirin-N, cpCV-N monomer 102 residues - 11013 Da.
| 1 | GLY | LEU | GLY | LYS | PHE | ILE | GLU | THR | CYS | ARG | ||||
| 2 | ASN | THR | GLN | LEU | ALA | GLY | SER | SER | GLU | LEU | ||||
| 3 | ALA | ALA | GLU | CYS | LYS | THR | ARG | ALA | GLN | GLN | ||||
| 4 | PHE | VAL | SER | THR | LYS | ILE | ASN | LEU | ASP | ASP | ||||
| 5 | HIS | ILE | ALA | ASN | ILE | ASP | GLY | THR | LEU | LYS | ||||
| 6 | TRP | GLN | PRO | SER | ASN | PHE | SER | GLN | THR | CYS | ||||
| 7 | TYR | ASN | SER | ALA | ILE | GLN | GLY | SER | VAL | LEU | ||||
| 8 | THR | SER | THR | CYS | GLU | ARG | THR | ASN | GLY | GLY | ||||
| 9 | TYR | ASN | THR | SER | SER | ILE | ASP | LEU | ASN | SER | ||||
| 10 | VAL | ILE | GLU | ASN | VAL | ASP | GLY | SER | LEU | LYS | ||||
| 11 | TYR | GLU |
Samples:
sample_1: Cyanovirin-N, [U-15N], 0.3 – 1 mM; phosphate buffer 20 mM
sample_2: Cyanovirin-N, [U-15N; U-13C], 0.3 – 1.0 mM; phosphate buffer 20 mM
Overall_conditions: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D 1H-13C HSQC/HMQC | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 3D DIPSI-H(CCO)NH | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D 15N-edited NOESY | not available | not available | not available |
| 4D 13C,13C NOESY | not available | not available | not available |
Software:
NMRView v4.1.1 -
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts