BMRB Entry 4906
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4906
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Title: Solution Structure of a C-Terminal Coiled-Coil Domain from Bovine IF1 - the Inhibitor Protein of F1 ATPase
Deposition date: 2000-11-30 Original release date: 2001-05-09
Authors: Gordon-Smith, Duncan; Carbajo, Rodrigo; Yang, Ji-Chung; Hortense, Videler; Runswick, Michael; Walker, John; Neuhaus, David
Citation: Gordon-Smith, Duncan; Carbajo, Rodrigo; Yang, Ji-Chun; Videler, Hortense; Runswick, Michael; Walker, John; Neuhaus, David. "Solution Structure of a C-terminal Coiled-coil Domain from Bovine IF1: The Inhibitor Protein of F1 ATPase" J. Mol. Biol. 308, 325-339 (2001).
Assembly members:
Inhibitor of F1 ATPase, polymer, 41 residues, 4984 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Inhibitor of F1 ATPase: ALKKHHENEISHHAKEIERL
QKEIERHKQSIKKLKQSEDD
D
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 306 |
| 13C chemical shifts | 180 |
| 15N chemical shifts | 54 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IF1 44-84 subunit 1 | 1 |
| 2 | IF1 44-84 subunit 2 | 1 |
Entities:
Entity 1, IF1 44-84 subunit 1 41 residues - 4984 Da.
| 1 | ALA | LEU | LYS | LYS | HIS | HIS | GLU | ASN | GLU | ILE | ||||
| 2 | SER | HIS | HIS | ALA | LYS | GLU | ILE | GLU | ARG | LEU | ||||
| 3 | GLN | LYS | GLU | ILE | GLU | ARG | HIS | LYS | GLN | SER | ||||
| 4 | ILE | LYS | LYS | LEU | LYS | GLN | SER | GLU | ASP | ASP | ||||
| 5 | ASP |
Samples:
sample_1: Inhibitor of F1 ATPase, [U-13C; U-15N], 1.5 mM
Ex-cond_1: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H COSY | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 15N NOESY HMQC | not available | not available | not available |
| 15N HMQC NOESY HMQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| 13C HCCH TOCSY | not available | not available | not available |
| 13C HSQC NOESY | not available | not available | not available |
| 13C,15N double half filtered NOESY | not available | not available | not available |
| 13C edit/purge HSQC NOESY | not available | not available | not available |
Software:
FELIX v97, Byosim Technologies - data analysis
X-WINNMR v2.1, Bruker - acquisition of spectra, processing of spectra
X-PLOR v3.1 - Structure calculations
NMR spectrometers:
- Bruker DRX 300 MHz
- Bruker AMX 500 MHz
- Bruker DRX 500 MHz
- Bruker DMX 600 MHz
- Bruker DMX 800 MHz
Related Database Links:
| PDB | |
| GB | AAA30396 AAI11646 ELR56311 |
| PRF | 1009211A |
| REF | NP_787010 XP_004023375 XP_005677044 XP_005895981 XP_005960342 |
| SP | P01096 |
| TPG | DAA32068 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts