BMRB Entry 4682
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4682
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Title: Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for holo-CRBP II PubMed: 10884357
Deposition date: 2000-03-06 Original release date: 2000-08-03
Authors: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen
Citation: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen. "Binding of retinol induces changes in rat cellular retinol-binding protein II conformation and backbone dynamics" J. Mol. Biol. 300, 619-632 (2000).
Assembly members:
Holo cellular retinol-binding protein II, polymer, 134 residues, 286.452 Da.
RTL, non-polymer, 286.452 Da.
Natural source: Common Name: norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Holo cellular retinol-binding protein II: MTKDQNGTWEMESNENFEGY
MKALDIDFATRKIAVRLTQT
KIIVQDGDNFKTKTNSTFRN
YDLDFTVGVEFDEHTKGLDG
RNVKTLVTWEGNTLVCVQKG
EKENRGWKQWVEGDKLYLEL
TCGDQVCRQVFKKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 544 |
| 1H chemical shifts | 1496 |
| 15N chemical shifts | 304 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Holo CRBP II | 1 |
| 2 | RTL | 2 |
Entities:
Entity 1, Holo CRBP II 134 residues - 286.452 Da.
| 1 | MET | THR | LYS | ASP | GLN | ASN | GLY | THR | TRP | GLU | ||||
| 2 | MET | GLU | SER | ASN | GLU | ASN | PHE | GLU | GLY | TYR | ||||
| 3 | MET | LYS | ALA | LEU | ASP | ILE | ASP | PHE | ALA | THR | ||||
| 4 | ARG | LYS | ILE | ALA | VAL | ARG | LEU | THR | GLN | THR | ||||
| 5 | LYS | ILE | ILE | VAL | GLN | ASP | GLY | ASP | ASN | PHE | ||||
| 6 | LYS | THR | LYS | THR | ASN | SER | THR | PHE | ARG | ASN | ||||
| 7 | TYR | ASP | LEU | ASP | PHE | THR | VAL | GLY | VAL | GLU | ||||
| 8 | PHE | ASP | GLU | HIS | THR | LYS | GLY | LEU | ASP | GLY | ||||
| 9 | ARG | ASN | VAL | LYS | THR | LEU | VAL | THR | TRP | GLU | ||||
| 10 | GLY | ASN | THR | LEU | VAL | CYS | VAL | GLN | LYS | GLY | ||||
| 11 | GLU | LYS | GLU | ASN | ARG | GLY | TRP | LYS | GLN | TRP | ||||
| 12 | VAL | GLU | GLY | ASP | LYS | LEU | TYR | LEU | GLU | LEU | ||||
| 13 | THR | CYS | GLY | ASP | GLN | VAL | CYS | ARG | GLN | VAL | ||||
| 14 | PHE | LYS | LYS | LYS |
Entity 2, RTL - C20 H30 O - 286.452 Da.
| 1 | RTL |
Samples:
sample_1: Holo cellular retinol-binding protein II, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM
sample_2: Holo cellular retinol-binding protein II 1.5 mM
sample_3: Holo cellular retinol-binding protein II, [U-99% 15N], 1.5 mM
Ex-con_1: pH: 7.4; temperature: 298 K; ionic strength: 0.081 M
Ex-con_2: pH: 6.5; temperature: 298 K; ionic strength: 0.079 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz
- Varian UnityPlus 500 MHz