BMRB Entry 4652
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4652
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Title: Solution Structure of Amino Terminus of Bovine Rhodopsin (residues 1-40) PubMed: 10888202
Deposition date: 2000-01-28 Original release date: 2001-05-09
Authors: Yeagle, P.; Salloum, A.; Chopra, A.; Bhawsar, N.; Ali, L.
Citation: Yeagle, P.; Salloum, A.; Chopra, A.; Bhawsar, N.; Ali, L.; Kuzmanovski, G.; Alderfer, J.; Albert, A.. "Structures of the intradiskal loops and amino terminus of the G-protein receptor, rhodopsin" J. Pept. Res. 55, 455-465 (2000).
Assembly members:
Rhodopsin, polymer, 40 residues, Formula weight is not available
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Rhodopsin: MNGTEGPNFYVPFSNKTGVV
RSPFEAPQYYLAEPWEFSML
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 253 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rhodopsin | 1 |
Entities:
Entity 1, Rhodopsin 40 residues - Formula weight is not available
| 1 | MET | ASN | GLY | THR | GLU | GLY | PRO | ASN | PHE | TYR | |
| 2 | VAL | PRO | PHE | SER | ASN | LYS | THR | GLY | VAL | VAL | |
| 3 | ARG | SER | PRO | PHE | GLU | ALA | PRO | GLN | TYR | TYR | |
| 4 | LEU | ALA | GLU | PRO | TRP | GLU | PHE | SER | MET | LEU |
Samples:
sample_1: Rhodopsin 2 mM; DMSO%
sample_cond_1: pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
Software:
FELIX - data analysis
DIANA - structure solution
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB83621 |
| GB | AAA30674 AAA30675 ELR51227 |
| PRF | 0811197A 0901188A 0901212A 0906201A 1001148A |
| REF | NP_001014890 XP_004018583 XP_005902896 XP_005955807 XP_010860750 |
| SP | P02699 P02700 |
| TPG | DAA16827 |