BMRB Entry 4628
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4628
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Heavy Chain Variable domain from LLama
Deposition date: 2000-11-09 Original release date: 2004-12-07
Authors: Renisio, J.-G.; Perez, J.; Czisch, M.; Guenneugues, M.; Bornet, O.; Frenken, L.; Cambillau, C.; Darbon, H.
Citation: Renisio, J.-G.; Perez, J.; Czisch, M.; Guenneugues, M.; Bornet, O.; Frenken, L.; Cambillau, C.; Darbon, H.. "Solution structure and backbone dynamics of an antigen-free heavy chain variable domain (VHH) from Llama" Proteins: Struct., Funct., Genet. 47, 546-555 (2002).
Assembly members:
H14, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: llama Taxonomy ID: 9844 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lama glama
Experimental source: Production method: .
Entity Sequences (FASTA):
H14: QVQLQESGGGLVQAGGSLRL
SCAASGRTGSTYDMGWFRQA
PGKERESVAAINWDSARTYY
ASSVRGRFTISRDNAKKTVY
LQMNSLKPEDTAVYTCGAGE
GGTWDSWGQGTQVTVSS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 679 |
| coupling constants | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H14 | 1 |
Entities:
Entity 1, H14 117 residues - Formula weight is not available
| 1 | GLN | VAL | GLN | LEU | GLN | GLU | SER | GLY | GLY | GLY | ||||
| 2 | LEU | VAL | GLN | ALA | GLY | GLY | SER | LEU | ARG | LEU | ||||
| 3 | SER | CYS | ALA | ALA | SER | GLY | ARG | THR | GLY | SER | ||||
| 4 | THR | TYR | ASP | MET | GLY | TRP | PHE | ARG | GLN | ALA | ||||
| 5 | PRO | GLY | LYS | GLU | ARG | GLU | SER | VAL | ALA | ALA | ||||
| 6 | ILE | ASN | TRP | ASP | SER | ALA | ARG | THR | TYR | TYR | ||||
| 7 | ALA | SER | SER | VAL | ARG | GLY | ARG | PHE | THR | ILE | ||||
| 8 | SER | ARG | ASP | ASN | ALA | LYS | LYS | THR | VAL | TYR | ||||
| 9 | LEU | GLN | MET | ASN | SER | LEU | LYS | PRO | GLU | ASP | ||||
| 10 | THR | ALA | VAL | TYR | THR | CYS | GLY | ALA | GLY | GLU | ||||
| 11 | GLY | GLY | THR | TRP | ASP | SER | TRP | GLY | GLN | GLY | ||||
| 12 | THR | GLN | VAL | THR | VAL | SER | SER |
Samples:
sample_1: H14, [U-15N], 3 mM; Na acetate, [U-2H], 10 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 4.6; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
Software:
UXNMR v2.6 - data collection, data processing
xeasy v1.3.13 - data analysis
diana v2.8 - structure solution
cns v1.0 - refinement
NMR spectrometers:
- Bruker DRX 500 MHz