BMRB Entry 4496
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4496
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution nmr structure of the mitochondrial protein import receptor Tom20 from rat in a complex with a presequence peptide derived from rat aldehyde dehydrogenase (ALDH) PubMed: 10721992
Deposition date: 1999-04-24 Original release date: 2000-03-23
Authors: Abe, Y.; Shodai, T.; Muto, T.; Mihara, K.; Torii, H.; Nishikawa, S.; Endo, T.; Kohda, D.
Citation: Abe, Y.; Shodai, T.; Muto, T.; Mihara, K.; Torii, H.; Nishikawa, S.; Endo, T.; Kohda, D.. "Structure Basis of Presequence Recognition by the Mitochondrial Protein Import Receptor Tom20" Cell 100, 551-560 (2000).
Assembly members:
Tom20, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Tom20: RAGLSKLPDLKDAEAVQKFF
LEEIQLGEELLAQGDYEKGV
DHLTNAIAVCGQPQQLLQVL
QQTLPPPVFQMLLTKLPTIS
QRIVSAQSLGEDDVE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 711 |
| 13C chemical shifts | 416 |
| 15N chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tom20 | 1 |
Entities:
Entity 1, Tom20 95 residues - Formula weight is not available
| 1 | ARG | ALA | GLY | LEU | SER | LYS | LEU | PRO | ASP | LEU | ||||
| 2 | LYS | ASP | ALA | GLU | ALA | VAL | GLN | LYS | PHE | PHE | ||||
| 3 | LEU | GLU | GLU | ILE | GLN | LEU | GLY | GLU | GLU | LEU | ||||
| 4 | LEU | ALA | GLN | GLY | ASP | TYR | GLU | LYS | GLY | VAL | ||||
| 5 | ASP | HIS | LEU | THR | ASN | ALA | ILE | ALA | VAL | CYS | ||||
| 6 | GLY | GLN | PRO | GLN | GLN | LEU | LEU | GLN | VAL | LEU | ||||
| 7 | GLN | GLN | THR | LEU | PRO | PRO | PRO | VAL | PHE | GLN | ||||
| 8 | MET | LEU | LEU | THR | LYS | LEU | PRO | THR | ILE | SER | ||||
| 9 | GLN | ARG | ILE | VAL | SER | ALA | GLN | SER | LEU | GLY | ||||
| 10 | GLU | ASP | ASP | VAL | GLU |
Samples:
sample_1: Tom20, [U-13C; U-15N], 0.5 mM
sample_cond_1: pH: 6.8; temperature: 303 K; ionic strength: 0.02 M; pressure: 1 atm
sample_cond_2: pH: 5.4; temperature: 303 K; ionic strength: 0.02 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA, HN(CO)CA | sample_1 | not available | not available |
| CBCA(CO)NH | sample_1 | not available | not available |
| HNCACB | sample_1 | not available | not available |
| HBHA(CO)NH | sample_1 | not available | not available |
| C(CO)NH-TOCSY HC(CO)NH-TOCSY | sample_1 | not available | not available |
| HCCH-COSY, HCCH-TOCSY | sample_1 | not available | not available |
| 15N-resolved NOESY | sample_1 | not available | not available |
| 15N-resolved TOCSY | sample_1 | not available | not available |
| NOESY | sample_1 | not available | not available |
| TOCSY | sample_1 | not available | not available |
| HNHA | sample_1 | not available | not available |
| {1H}-15N NOE | sample_1 | not available | not available |
| 3D F1 13C-filtered-F3 13C-resolved NOESY | sample_1 | not available | not available |
| 2D F1/F2 13C-filtered NOESY | sample_1 | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAA02804 BAA09714 BAB22444 BAE24663 BAE35669 |
| EMBL | CAH91379 |
| GB | AAB01506 AAB35420 AAF13354 AAH00882 AAH02087 |
| REF | NP_001092653 NP_001127411 NP_001232519 NP_001247980 NP_055580 |
| SP | A6H7B1 Q15388 Q5RA31 Q62760 Q9DCC8 |
| TPG | DAA14365 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts