BMRB Entry 4429
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4429
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Title: 1H and 15N Chemical Shift Assignments for ribosomal protein L7
Deposition date: 1999-10-03 Original release date: 2002-07-12
Authors: Bocharov, Eduard
Citation: Bocharov, Eduard; Gudkov, Anatolij; Arseniev, Alexandr. "Topology of the secondary structure elements of ribosomal protein L7/L12 from E.coli in solutio" FEBS Lett. 379, 291-294 (1996).
Assembly members:
E.coli ribosomal protein L7/L12, polymer, 120 residues, 12164 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
E.coli ribosomal protein L7/L12: SITKDQIIEAVAAMSVMDVV
ELISAMEEKFGVSAAAAVAV
AAGPVEAAEEKTEFDVILKA
AGANKVAVIKAVRGATGLGL
KEAKDLVESAPAALKEGVSK
DDAEALKKALEEAGAEVEVK
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 823 |
| 15N chemical shifts | 120 |
| coupling constants | 118 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L7 dimer subunit A | 1 |
| 2 | L7 dimer subunit B | 1 |
Entities:
Entity 1, L7 dimer subunit A 120 residues - 12164 Da.
| 1 | SER | ILE | THR | LYS | ASP | GLN | ILE | ILE | GLU | ALA | |
| 2 | VAL | ALA | ALA | MET | SER | VAL | MET | ASP | VAL | VAL | |
| 3 | GLU | LEU | ILE | SER | ALA | MET | GLU | GLU | LYS | PHE | |
| 4 | GLY | VAL | SER | ALA | ALA | ALA | ALA | VAL | ALA | VAL | |
| 5 | ALA | ALA | GLY | PRO | VAL | GLU | ALA | ALA | GLU | GLU | |
| 6 | LYS | THR | GLU | PHE | ASP | VAL | ILE | LEU | LYS | ALA | |
| 7 | ALA | GLY | ALA | ASN | LYS | VAL | ALA | VAL | ILE | LYS | |
| 8 | ALA | VAL | ARG | GLY | ALA | THR | GLY | LEU | GLY | LEU | |
| 9 | LYS | GLU | ALA | LYS | ASP | LEU | VAL | GLU | SER | ALA | |
| 10 | PRO | ALA | ALA | LEU | LYS | GLU | GLY | VAL | SER | LYS | |
| 11 | ASP | ASP | ALA | GLU | ALA | LEU | LYS | LYS | ALA | LEU | |
| 12 | GLU | GLU | ALA | GLY | ALA | GLU | VAL | GLU | VAL | LYS |
Samples:
sample_1: E.coli ribosomal protein L7/L12, [U-95% 15N], 1.0 mM
sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HMQC | sample_1 | not available | sample_cond_1 |
| 1H-15N HSQC | sample_1 | not available | sample_cond_1 |
| 1H-15N TOCSY-HSQC | sample_1 | not available | sample_cond_1 |
| 1H-15N NOESY-HSQC | sample_1 | not available | sample_cond_1 |
| 1H TOCSY | sample_1 | not available | sample_cond_1 |
| 1H NOESY | sample_1 | not available | sample_cond_1 |
Software:
XEASY v1.2.11 - peak assignments
DYANA v1.5 - structure calculation
FANTOM v4 - energy minimization
MOLMOL v2.5.1 - visualization and structure analysis
NMR spectrometers:
- Varian UNITY 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB38332 BAE77334 BAG79797 BAH61123 BAI28246 |
| EMBL | CAA23624 CAA37246 CAD09488 CAP78442 CAQ34332 |
| GB | AAC43084 AAC76960 AAF33498 AAG59182 AAL22980 |
| PIR | AF0933 |
| PRF | 0601198A |
| REF | NP_312936 NP_418413 NP_457918 NP_463021 NP_709781 |
| SP | A6TGN9 A7ZUK0 A8A785 A8AKU0 A9N0J3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts