BMRB Entry 4312
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4312
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Title: 1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF
Deposition date: 1999-02-28 Original release date: 1999-03-03
Authors: Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming
Citation: Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming. "1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF" J. Biomol. NMR ., .-..
Assembly members:
histone acetyltransferase bromodomain, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
histone acetyltransferase bromodomain: GSHMSKEPRDPDQLYSTLKS
ILQQVKSHQSAWPFMEPVKR
TEAPGYYEVIRSPMDLKTMS
ERLKNRYYVSKKLFMADLQR
VFTNCKEYNAPESEYYKCAN
ILEKFFFSKIKEAGLIDK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 382 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 708 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | brd subunit 1 | 1 |
Entities:
Entity 1, brd subunit 1 118 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | SER | LYS | GLU | PRO | ARG | ASP | ||||
| 2 | PRO | ASP | GLN | LEU | TYR | SER | THR | LEU | LYS | SER | ||||
| 3 | ILE | LEU | GLN | GLN | VAL | LYS | SER | HIS | GLN | SER | ||||
| 4 | ALA | TRP | PRO | PHE | MET | GLU | PRO | VAL | LYS | ARG | ||||
| 5 | THR | GLU | ALA | PRO | GLY | TYR | TYR | GLU | VAL | ILE | ||||
| 6 | ARG | SER | PRO | MET | ASP | LEU | LYS | THR | MET | SER | ||||
| 7 | GLU | ARG | LEU | LYS | ASN | ARG | TYR | TYR | VAL | SER | ||||
| 8 | LYS | LYS | LEU | PHE | MET | ALA | ASP | LEU | GLN | ARG | ||||
| 9 | VAL | PHE | THR | ASN | CYS | LYS | GLU | TYR | ASN | ALA | ||||
| 10 | PRO | GLU | SER | GLU | TYR | TYR | LYS | CYS | ALA | ASN | ||||
| 11 | ILE | LEU | GLU | LYS | PHE | PHE | PHE | SER | LYS | ILE | ||||
| 12 | LYS | GLU | ALA | GLY | LEU | ILE | ASP | LYS |
Samples:
sample_1: histone acetyltransferase bromodomain, [U-13C; U-15N; U-75% 2H], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%
sample_2: histone acetyltransferase bromodomain, [U-13C; U-15N], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%
Ex-cond_1: pH: 6.5 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | Ex-cond_1 |
| 2D 1H-13C HSQC | not available | not available | Ex-cond_1 |
| 3D HNCACB | not available | not available | Ex-cond_1 |
| 3D HN(CO)CACB | not available | not available | Ex-cond_1 |
| 3D (H)C(CO)NH-TOCSY | not available | not available | Ex-cond_1 |
| 3D HNHA | not available | not available | Ex-cond_1 |
| 3D HCCH TOCSY | not available | not available | Ex-cond_1 |
| 15N edited TOCSY | not available | not available | Ex-cond_1 |
| 15N edited NOESY | not available | not available | Ex-cond_1 |
| 13C edited NOESY | not available | not available | Ex-cond_1 |
Software:
NMRPipe v3.1 - spectra Processing
NMRVIEW v3.1 - spectra analysis
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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