BMRB Entry 4246
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4246
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 3D Structure of the M8L Mutant of Squash Trypsin Inhibitor CMTI-I, NMR, 6 Structures
Deposition date: 1998-10-15 Original release date: 2000-05-02
Authors: Zhukov, Igor; Bolewska, Krystina; Bierzynski, Andrzej
Citation: Zhukov, Igor; Bolewska, Krystina; Bierzynski, Andrzej. "Conservative Mutation Met8-->Leu Affects the Folding Process and Structural Stability of Squash Trypsin Inhibitor CMTI-I" Protein Sci. 9, 273-279 (2000).
Assembly members:
CMTI-I(M8L), polymer, 29 residues, Formula weight is not available
Natural source: Common Name: squash Taxonomy ID: 3661 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Cucurbita maxima
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CMTI-I(M8L): RVCPRILLECKKDSDCLAEC
VCLEHGYCG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 198 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CMTI-I(M8L) | 1 |
Entities:
Entity 1, CMTI-I(M8L) 29 residues - Formula weight is not available
| 1 | ARG | VAL | CYS | PRO | ARG | ILE | LEU | LEU | GLU | CYS | ||||
| 2 | LYS | LYS | ASP | SER | ASP | CYS | LEU | ALA | GLU | CYS | ||||
| 3 | VAL | CYS | LEU | GLU | HIS | GLY | TYR | CYS | GLY |
Samples:
sample_one: CMTI-I(M8L) mM; NaCl 50 mM
sample_conditions_one: pH: 5.6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
| DQF-COSY | sample_one | not available | sample_conditions_one |
Software:
XEASY v3.21 - assignment and integration of two-dimensional NMR spectra
NMR spectrometers:
- Varian UNITYplus 500 MHz
Related Database Links:
| PDB |