BMRB Entry 4188
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4188
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Title: C2 Domain of Cytosolic Phospholipase A2
Deposition date: 1998-08-18 Original release date: 2000-03-08
Authors: Xu, Guang-Yi; McDonagh, Thomas; Yu, Hsiang-Ai; Nalefski, Eric; Clark, James; Cumming, Dale
Citation: Xu, Guang-Yi; McDonagh, Thomas; Yu, Hsiang-Ai; Nalefski, Eric; Clark, James; Cumming, Dale. "Solution Structure and Membrane Interactions of the C2 Domain of Cytosolic Phospholipase A2" J. Mol. Biol. 280, 485-500 (1998).
Assembly members:
C2 Domain of Cytosolic Phospholipase A2, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eucaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
C2 Domain of Cytosolic Phospholipase A2: MSFIDPYQHIIVEHQYSHKF
TVVVLRATKVTKGAFGDMLD
TPDPYVELFISTTPDSRKRT
RHFNNDINPVWNETFEFILD
PNQENVLEITLMDANYVMDE
TLGTATFTVSSMKVGEKKEV
PFIFNQVTEMVLEMSLEV
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 576 |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 969 |
| coupling constants | 99 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cPLA2-C2 | 1 |
Entities:
Entity 1, cPLA2-C2 138 residues - Formula weight is not available
| 1 | MET | SER | PHE | ILE | ASP | PRO | TYR | GLN | HIS | ILE | ||||
| 2 | ILE | VAL | GLU | HIS | GLN | TYR | SER | HIS | LYS | PHE | ||||
| 3 | THR | VAL | VAL | VAL | LEU | ARG | ALA | THR | LYS | VAL | ||||
| 4 | THR | LYS | GLY | ALA | PHE | GLY | ASP | MET | LEU | ASP | ||||
| 5 | THR | PRO | ASP | PRO | TYR | VAL | GLU | LEU | PHE | ILE | ||||
| 6 | SER | THR | THR | PRO | ASP | SER | ARG | LYS | ARG | THR | ||||
| 7 | ARG | HIS | PHE | ASN | ASN | ASP | ILE | ASN | PRO | VAL | ||||
| 8 | TRP | ASN | GLU | THR | PHE | GLU | PHE | ILE | LEU | ASP | ||||
| 9 | PRO | ASN | GLN | GLU | ASN | VAL | LEU | GLU | ILE | THR | ||||
| 10 | LEU | MET | ASP | ALA | ASN | TYR | VAL | MET | ASP | GLU | ||||
| 11 | THR | LEU | GLY | THR | ALA | THR | PHE | THR | VAL | SER | ||||
| 12 | SER | MET | LYS | VAL | GLY | GLU | LYS | LYS | GLU | VAL | ||||
| 13 | PRO | PHE | ILE | PHE | ASN | GLN | VAL | THR | GLU | MET | ||||
| 14 | VAL | LEU | GLU | MET | SER | LEU | GLU | VAL |
Samples:
sample_one: C2 Domain of Cytosolic Phospholipase A2, [U-95% 13C; U-95% 15N], 0.5 mM
sample_conditions_one: ionic strength: 0.0 M; pH*: 7.1; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | not available |
| TOCSY | sample_one | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Unity Plus 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE26047 BAF83025 BAG64096 BAI46198 |
| EMBL | CAH92005 |
| GB | AAA60105 AAB00789 AAB00796 AAB33847 AAC21591 |
| PRF | 2022317A |
| REF | NP_001075312 NP_001126164 NP_001298122 NP_032895 NP_077734 |
| SP | O77793 P47712 P47713 P50393 Q5R8A5 |
Download simulated HSQC data in one of the following formats:
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