BMRB Entry 4155
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4155
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Title: Solution Structure of Eotaxin: A Chemokine that Selectively Recruits Eosinophils in Allergic Inflammation
Deposition date: 1998-06-29 Original release date: 1999-03-01
Authors: Crump, M.; Rajarathnam, K.; Kim, K.-S; Sykes, B.
Citation: Crump, M.; Rajarathnam, K.; Kim, K.-S; Sykes, B.. "Solution Structure of Eotaxin: a Chemokine That Selectively Recruits Eosinophils in Allergic Inflammation" J. Biol. Chem. 273, 22471-22479 (1998).
Assembly members:
Eotaxin, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Eotaxin: XPASVPTTCCFNLANRKIPL
QRLESYRRITSGKCPQKAVI
FKTKLAKDICADPKKKWVQD
SMKYLDQKSPTPKP
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 478 |
| coupling constants | 56 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EOT | 1 |
Entities:
Entity 1, EOT 74 residues - Formula weight is not available
| 1 | PCA | PRO | ALA | SER | VAL | PRO | THR | THR | CYS | CYS | ||||
| 2 | PHE | ASN | LEU | ALA | ASN | ARG | LYS | ILE | PRO | LEU | ||||
| 3 | GLN | ARG | LEU | GLU | SER | TYR | ARG | ARG | ILE | THR | ||||
| 4 | SER | GLY | LYS | CYS | PRO | GLN | LYS | ALA | VAL | ILE | ||||
| 5 | PHE | LYS | THR | LYS | LEU | ALA | LYS | ASP | ILE | CYS | ||||
| 6 | ALA | ASP | PRO | LYS | LYS | LYS | TRP | VAL | GLN | ASP | ||||
| 7 | SER | MET | LYS | TYR | LEU | ASP | GLN | LYS | SER | PRO | ||||
| 8 | THR | PRO | LYS | PRO |
Samples:
sample_one: Eotaxin, [U-15N], 1.0 mM; sodium azide 1.0 mM; sodium acetate 20.0 mM; DSS 1.0 mM
sample_conditions_one: pH: 5.0 na; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
| NOESY | sample_one | not available | sample_conditions_one |
| ROESY | sample_one | not available | sample_conditions_one |
| 13C-HSQC (natural abundance) | sample_one | not available | sample_conditions_one |
| 1H-15N HSQC | not available | $sample_one | not available |
| 7 | not available | $sample_one | not available |
| 8 | not available | $sample_one | not available |
| 9 | not available | $sample_one | not available |
| 10 | not available | $sample_one | not available |
| 11 | not available | $sample_one | not available |
| 12 | not available | $sample_one | not available |
| not available | not available | not available | not available |
Software:
nmrPipe/PIPP v4.2 - Peak Picking of 15N edited TOCSY and 15N edited NOESY spectra
VNMR v5.3 Revision B - Peak Picking of COSY, NOESY and TOCSY spectra of unlabelled samples
NMR spectrometers:
- Varian Unity 600 MHz
- Varian Inova 500 MHz
Related Database Links:
| BMRB | 19989 4390 |
| PDB | |
| DBJ | BAA08370 BAG72995 |
| EMBL | CAA99997 CAA99998 CAB07027 CAG33702 |
| GB | AAA98957 AAC50369 AAC51297 AAH17850 ABK41951 |
| PRF | 2208449A |
| REF | NP_002977 XP_002827294 XP_003818054 XP_004041989 XP_004041990 |
| SP | P51671 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts