BMRB Entry 36343
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36343
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Title: a new lasso peptide koreensin
Deposition date: 2020-04-13 Original release date: 2020-09-07
Authors: Hemmi, H.; Kodani, S.
Citation: Fuwa, H.; Hemmi, H.; Kaweewan, I.; Kodani, S.. "heterologous production of a new lasso peptide koreensin based on genome mining" . ., .-..
Assembly members:
lasso peptide koreensin, polymer, 17 residues, 1672.839 Da.
Natural source: Common Name: Sphingomonas koreensis Taxonomy ID: 93064 Superkingdom: Bacteria Kingdom: not available Genus/species: Sphingomonas koreensis
Experimental source: Production method: recombinant technology Host organism: Novosphingobium subterraneum
Entity Sequences (FASTA):
lasso peptide koreensin: GPKGDFPDVGDGRILAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 71 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 17 residues - 1672.839 Da.
| 1 | GLY | PRO | LYS | GLY | ASP | PHE | PRO | ASP | VAL | GLY | ||||
| 2 | ASP | GLY | ARG | ILE | LEU | ALA | GLY |
Samples:
sample_1: koreensin 3.0 mg/mL; DMSO-d6, [U-2H], 100%
sample_conditions_1: ionic strength: 0 Not defined; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 1D 13C | sample_1 | isotropic | sample_conditions_1 |
| DEPT-135 | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMBC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
Sparky, Goddard - chemical shift assignment, peak picking
TopSpin, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker AVANCE 600 MHz
- Bruker AVANCE III 800 MHz