BMRB Entry 36012
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36012
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Title: Solution structure of nedd8 from Trypanosoma brucei
Deposition date: 2016-07-25 Original release date: 2017-07-24
Authors: Wang, R.; Liao, S.; Zhang, J.; Tu, X.
Citation: Wang, R.; Liao, S.; Zhang, J.; Tu, X.. "Solution structure of nedd8 from Trypanosoma brucei" . ., .-..
Assembly members:
entity_1, polymer, 86 residues, 9697.252 Da.
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 185431 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Entity Sequences (FASTA):
entity_1: MGHHHHHHMLLKVKTVSNKV
IQITSLTDDNTIAELKGKLE
ESEGIPGNMIRLVYQGKQLE
DEKRLKDYQMSAGATFHMVV
ALRAGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 223 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 479 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 86 residues - 9697.252 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | MET | LEU | ||||
| 2 | LEU | LYS | VAL | LYS | THR | VAL | SER | ASN | LYS | VAL | ||||
| 3 | ILE | GLN | ILE | THR | SER | LEU | THR | ASP | ASP | ASN | ||||
| 4 | THR | ILE | ALA | GLU | LEU | LYS | GLY | LYS | LEU | GLU | ||||
| 5 | GLU | SER | GLU | GLY | ILE | PRO | GLY | ASN | MET | ILE | ||||
| 6 | ARG | LEU | VAL | TYR | GLN | GLY | LYS | GLN | LEU | GLU | ||||
| 7 | ASP | GLU | LYS | ARG | LEU | LYS | ASP | TYR | GLN | MET | ||||
| 8 | SER | ALA | GLY | ALA | THR | PHE | HIS | MET | VAL | VAL | ||||
| 9 | ALA | LEU | ARG | ALA | GLY | CYS |
Samples:
sample_1: entity_1 mM; Dithiothreitol 2 mM; Ethylene Diamine Tetraacetic Acid 2 uM; Sodium dihydrogen phosphate 20 mM; arginine 25 uM; glutamine 25 uM; sodium chloride 150 uM; D2O, [U-2H], 100%
sample_conditions_1: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DMX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts