BMRB Entry 30488

Name: Solution structure of Rbfox2 RRM mimetic peptide CPfox5
Published: 2019-05-07

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PDB ID: 6dzb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30488
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of Rbfox2 RRM mimetic peptide CPfox5 PubMed: 30537200

Deposition date: 2018-07-03 Original release date: 2019-05-07

Authors: Sun, Y.-T.; Varani, G.

Citation: Sun, Yi-Ting; Shortridge, Matthew; Varani, Gabriele. "A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b." Chembiochem 20, 931-939 (2019).

Assembly members:
entity_1, polymer, 16 residues, 1925.241 Da.

Natural source: Common Name: not available Taxonomy ID: 36630 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GSRGFRFXPKIIFNER

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	14
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 16 residues - 1925.241 Da.

1

GLY

SER

ARG

GLY

PHE

ARG

PHE

DPR

PRO

LYS

2

ILE

PHE

ASN

GLU

ARG

Samples:

sample_1: CPfox5 4.0 mg/mL; potassium phosphate 10 mM; potassium chloride 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts