BMRB Entry 30119
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30119
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Connexin 26 WT peptide NMR Structure PubMed: 27378082
Deposition date: 2016-06-17 Original release date: 2016-09-23
Authors: Dowd, T.; Bargiello, T.
Citation: Batir, Y.; Bargiello, T.; Dowd, T.. "Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy." Arch. Biochem. Biophys. 608, 8-19 (2016).
Assembly members:
entity_1, polymer, 23 residues, 2372.720 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XMDWGTLQTILGGVNKHSTS
IGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 40 |
| 1H chemical shifts | 133 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 23 residues - 2372.720 Da.
| 1 | ACE | MET | ASP | TRP | GLY | THR | LEU | GLN | THR | ILE | ||||
| 2 | LEU | GLY | GLY | VAL | ASN | LYS | HIS | SER | THR | SER | ||||
| 3 | ILE | GLY | LYS |
Samples:
sample_1: entity_1 1.0 ± 0.1 mM; KCl 100 ± 0.1 mM; TSP 100 ± 2 uM
sample_conditions_2: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_2 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
VNMR, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 600 MHz