BMRB Entry 30031
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30031
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Title: Solution Structure of the Microtubule-Targeting COS Domain of MID1 PubMed: 27367845
Deposition date: 2016-03-05 Original release date: 2016-07-06
Authors: Wright, K.; Du, H.; Dagnachew, M.; Massiah, M.
Citation: Wright, K.; Du, H.; Dagnachew, M.; Massiha, M.. "Solution structure of the microtubule-targeting COS domain of MID1" FEBS J. 283, 3089-3102 (2016).
Assembly members:
entity_1, polymer, 60 residues, 6926.834 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Entity Sequences (FASTA):
entity_1: LKENDHARFLQTAKNITERV
SMATASSQVLIPEINLNDTF
DTFALDFSREKKLLECLDYL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 231 |
| 15N chemical shifts | 52 |
| 1H chemical shifts | 379 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 60 residues - 6926.834 Da.
| 1 | LEU | LYS | GLU | ASN | ASP | HIS | ALA | ARG | PHE | LEU | |
| 2 | GLN | THR | ALA | LYS | ASN | ILE | THR | GLU | ARG | VAL | |
| 3 | SER | MET | ALA | THR | ALA | SER | SER | GLN | VAL | LEU | |
| 4 | ILE | PRO | GLU | ILE | ASN | LEU | ASN | ASP | THR | PHE | |
| 5 | ASP | THR | PHE | ALA | LEU | ASP | PHE | SER | ARG | GLU | |
| 6 | LYS | LYS | LEU | LEU | GLU | CYS | LEU | ASP | TYR | LEU |
Samples:
sample_1: entity_1 mM; TRIS 50 mM; beta-mercaptoethanol 10 mM; sodium azide 0.2%
sample_conditions_1: ionic strength: 50 mM; pH: 7.8; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Agilent Agilent DD2 600 MHz 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts