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BMRB Entry 30030

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30030
MolProbity Validation Chart

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Title: Solution structure of AN1-type zinc finger domain from Cuz1 (Cdc48 associated ubiquitin-like/zinc-finger protein-1)   PubMed: 27662200

Deposition date: 2016-03-01 Original release date: 2016-09-30

Authors: Sun, Z.-Y; Hanna, J.; Wagner, G.; Bhanu, M.; Allan, M.; Arthanari, H.

Citation: Sun, Z.-Y; Bhanu, M.; Allan, M.; Arthanari, H.; Wagner, G.; Hanna, J.. "Solution Structure of the Cuz1 AN1 Zinc Finger Domain: An Exposed LDFLP Motif Defines a Subfamily of AN1 Proteins"  PLoS One 11, e0163660-e0163660 (2016).

Assembly members:
CDC48-associated ubiquitin-like/zinc finger protein 1, polymer, 49 residues, 6003.831 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: bakers yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces Cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CDC48-associated ubiquitin-like/zinc finger protein 1: MLDVGKHCAYCRQLDFLPFH CSFCNEDFCSNHRLKEDHHC RWLLEHEEV

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts62
1H chemical shifts337

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION2
3ZINC ION2

Entities:

Entity 1, entity_1 49 residues - 6003.831 Da.

1   METLEUASPVALGLYLYSHISCYSALATYR
2   CYSARGGLNLEUASPPHELEUPROPHEHIS
3   CYSSERPHECYSASNGLUASPPHECYSSER
4   ASNHISARGLEULYSGLUASPHISHISCYS
5   ARGTRPLEULEUGLUHISGLUGLUVAL

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: DTT 1 mM; NaCl 50 mM; Tris 5 mM; ZnCl2 0.2 mM; cuz1, [U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_2: DTT 1 mM; NaCl 50 mM; Tris 5 mM; ZnCl2 0.2 mM; cuz1, [U-100% 13C; U-100% 15N], 0.7 mM; H2O 90%; D2O 10%

sample_3: DTT, [U-2H], 1 mM; NaCl 50 mM; Tris, [U-2H], 5 mM; ZnCl2 0.2 mM; cuz1 0.85 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D NOESYsample_3isotropicsample_conditions_1
2D TOCSYsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

hmsIST, Hyberts, S.G and Wagner, G - processing

NMR spectrometers:

  • Agilent DD2 600 MHz
  • Bruker AvanceIII 750 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts