BMRB Entry 28028
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR28028
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Title: 1H, 13C, 15N backbone and side-chain assignment of the native form of UbcH7 (UBE2L3) PubMed: 31792831
Deposition date: 2019-10-09 Original release date: 2020-01-30
Authors: Marousis, Konstantinos; Seliami, Anastasia; Birkou, Maria; Spyroulias, Georgios
Citation: Marousis, Konstantinos; Birkou, Maria; Asimakopoulou, Antonia; Spyroulias, Georgios. "1H, 13C, 15N backbone and side-chain resonance assignment of the native form of UbcH7 (UBE2L3) through solution NMR spectroscopy" Biomol. NMR Assignments 14, 73-78 (2020).
Assembly members:
UbcH7, polymer, 154 residues, 17861.58 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
UbcH7: MAASRRLMKELEEIRKCGMK
NFRNIQVDEANLLTWQGLIV
PDNPPYDKGAFRIEINFPAE
YPFKPPKITFKTKIYHPNID
EKGQVCLPVISAENWKPATK
TDQVIQSLIALVNDPQPEHP
LRADLAEEYSKDRKKFCKNA
EEFTKKYGEKRPVD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 684 |
| 15N chemical shifts | 157 |
| 1H chemical shifts | 1116 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UbcH7 | 1 |
Entities:
Entity 1, UbcH7 154 residues - 17861.58 Da.
| 1 | MET | ALA | ALA | SER | ARG | ARG | LEU | MET | LYS | GLU | ||||
| 2 | LEU | GLU | GLU | ILE | ARG | LYS | CYS | GLY | MET | LYS | ||||
| 3 | ASN | PHE | ARG | ASN | ILE | GLN | VAL | ASP | GLU | ALA | ||||
| 4 | ASN | LEU | LEU | THR | TRP | GLN | GLY | LEU | ILE | VAL | ||||
| 5 | PRO | ASP | ASN | PRO | PRO | TYR | ASP | LYS | GLY | ALA | ||||
| 6 | PHE | ARG | ILE | GLU | ILE | ASN | PHE | PRO | ALA | GLU | ||||
| 7 | TYR | PRO | PHE | LYS | PRO | PRO | LYS | ILE | THR | PHE | ||||
| 8 | LYS | THR | LYS | ILE | TYR | HIS | PRO | ASN | ILE | ASP | ||||
| 9 | GLU | LYS | GLY | GLN | VAL | CYS | LEU | PRO | VAL | ILE | ||||
| 10 | SER | ALA | GLU | ASN | TRP | LYS | PRO | ALA | THR | LYS | ||||
| 11 | THR | ASP | GLN | VAL | ILE | GLN | SER | LEU | ILE | ALA | ||||
| 12 | LEU | VAL | ASN | ASP | PRO | GLN | PRO | GLU | HIS | PRO | ||||
| 13 | LEU | ARG | ALA | ASP | LEU | ALA | GLU | GLU | TYR | SER | ||||
| 14 | LYS | ASP | ARG | LYS | LYS | PHE | CYS | LYS | ASN | ALA | ||||
| 15 | GLU | GLU | PHE | THR | LYS | LYS | TYR | GLY | GLU | LYS | ||||
| 16 | ARG | PRO | VAL | ASP |
Samples:
sample_15N: UbcH7, [U-99% 15N], 1.0 mM; K2HPO4/KH2PO4 50.0 mM
sample_15N-13C: UbcH7, [U-98% 13C; U-98% 15N], 1.0 mM; K2HPO4/KH2PO4 50.0 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_15N | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_15N | isotropic | sample_conditions_1 |
| 3D HNHA | sample_15N | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HNCO | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HNCA | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_15N-13C | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_15N-13C | isotropic | sample_conditions_1 |
Software:
CARA v1.5.5, Keller and Wuthrich - chemical shift assignment
TOPSPIN v3.5 PL5, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance III HD 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts