BMRB Entry 26724
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26724
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Title: NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the presence of calcium PubMed: 27155947
Deposition date: 2015-12-28 Original release date: 2016-07-14
Authors: Kilpatrick, Adina; Gurrola, Theodore; Fowler, C. Andrew
Citation: Kilpatrick, Adina; Gurrola, Theodore; Sterner, Robert; Sleister, Heidi; Honts, Jerry; Fowler, C. Andrew. "Backbone and side-chain chemical shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila" Biomol. NMR Assign. ., .-. (2016).
Assembly members:
Tcb2-C, polymer, 102 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Tetrahymena thermophila Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena thermophila
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Tcb2-C: SSKPKYNPEVEAKLDVARRL
FKRYDKDGSGQLQDDEIAGL
LKDTYAEMGMSNFTPTKEDV
KIWLQMADTNSDGSVSLEEY
EDLIIKSLQKAGIRVEKQSL
VF
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 457 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 679 |
| T1 relaxation values | 90 |
| T2 relaxation values | 90 |
| heteronuclear NOE values | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tcb2-C | 1 |
| 2 | CALCIUM ION_1 | 2 |
| 3 | CALCIUM ION_2 | 2 |
Entities:
Entity 1, Tcb2-C 102 residues - Formula weight is not available
| 1 | SER | SER | LYS | PRO | LYS | TYR | ASN | PRO | GLU | VAL | ||||
| 2 | GLU | ALA | LYS | LEU | ASP | VAL | ALA | ARG | ARG | LEU | ||||
| 3 | PHE | LYS | ARG | TYR | ASP | LYS | ASP | GLY | SER | GLY | ||||
| 4 | GLN | LEU | GLN | ASP | ASP | GLU | ILE | ALA | GLY | LEU | ||||
| 5 | LEU | LYS | ASP | THR | TYR | ALA | GLU | MET | GLY | MET | ||||
| 6 | SER | ASN | PHE | THR | PRO | THR | LYS | GLU | ASP | VAL | ||||
| 7 | LYS | ILE | TRP | LEU | GLN | MET | ALA | ASP | THR | ASN | ||||
| 8 | SER | ASP | GLY | SER | VAL | SER | LEU | GLU | GLU | TYR | ||||
| 9 | GLU | ASP | LEU | ILE | ILE | LYS | SER | LEU | GLN | LYS | ||||
| 10 | ALA | GLY | ILE | ARG | VAL | GLU | LYS | GLN | SER | LEU | ||||
| 11 | VAL | PHE |
Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM
sample_2: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM
sample_3: Tcb2-C, [U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM
sample_4: Tcb2-C 1.1 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HACACO | sample_2 | isotropic | sample_conditions_1 |
| 2D (HB)CB(CGCD)HD | sample_2 | isotropic | sample_conditions_1 |
| 2D (HB)CB(CGCDCE)HE | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_1 |
| Heteronuclear NOE ratio | sample_1 | isotropic | sample_conditions_1 |
| T1 experiments | sample_1 | isotropic | sample_conditions_1 |
| T2 experiments | sample_1 | isotropic | sample_conditions_1 |
Software:
CccpNmr_Analysis, CCPN - chemical shift assignment, data analysis, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| NCBI | P09226.2 XP_001007754.3 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts