BMRB Entry 26553
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26553
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Title: Solution Structure of the Smarc Domain
Deposition date: 2015-04-10 Original release date: 2015-08-03
Authors: Allen, Mark; Freund, S.; Bycroft, M.
Citation: Allen, Mark; Freund, S.; Bycroft, M.. "Solution Structure of the Smarc Domain" To Be Published ., .-..
Assembly members:
SWI-SNF-RELATED_MATRIX-ASSOCIATED_ACTIN-DEPENDENT_OF_CHROMATIN_SUBFAMILY_B_M, polymer, 115 residues, 12981.9001 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SWI-SNF-RELATED_MATRIX-ASSOCIATED_ACTIN-DEPENDENT_OF_CHROMATIN_SUBFAMILY_B_M: GGSMMMALSKTFGQKPVKFQ
LEDDGEFYMIGSEVGNYLRM
FRGSLYKRYPSLWRRLATVE
ERKKIVASSHGKKTKPNTKD
HGYTTLATSVTLLKASEVEE
ILDGNDEKYKAVSIS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 333 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 828 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT OF CHROMATIN SUBFAMILY B M | 1 |
Entities:
Entity 1, SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT OF CHROMATIN SUBFAMILY B M 115 residues - 12981.9001 Da.
| 1 | GLY | GLY | SER | MET | MET | MET | ALA | LEU | SER | LYS | ||||
| 2 | THR | PHE | GLY | GLN | LYS | PRO | VAL | LYS | PHE | GLN | ||||
| 3 | LEU | GLU | ASP | ASP | GLY | GLU | PHE | TYR | MET | ILE | ||||
| 4 | GLY | SER | GLU | VAL | GLY | ASN | TYR | LEU | ARG | MET | ||||
| 5 | PHE | ARG | GLY | SER | LEU | TYR | LYS | ARG | TYR | PRO | ||||
| 6 | SER | LEU | TRP | ARG | ARG | LEU | ALA | THR | VAL | GLU | ||||
| 7 | GLU | ARG | LYS | LYS | ILE | VAL | ALA | SER | SER | HIS | ||||
| 8 | GLY | LYS | LYS | THR | LYS | PRO | ASN | THR | LYS | ASP | ||||
| 9 | HIS | GLY | TYR | THR | THR | LEU | ALA | THR | SER | VAL | ||||
| 10 | THR | LEU | LEU | LYS | ALA | SER | GLU | VAL | GLU | GLU | ||||
| 11 | ILE | LEU | ASP | GLY | ASN | ASP | GLU | LYS | TYR | LYS | ||||
| 12 | ALA | VAL | SER | ILE | SER |
Samples:
sample_1: SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT OF CHROMATIN SUBFAMILY B M, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 100.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
| TOCSY | sample_1 | solution | sample_conditions_1 |
| DQF-COSY | sample_1 | solution | sample_conditions_1 |
| HSQC | sample_1 | solution | sample_conditions_1 |
| HNCACB | sample_1 | solution | sample_conditions_1 |
| CBCACONH | sample_1 | solution | sample_conditions_1 |
| HNCO | sample_1 | solution | sample_conditions_1 |
| HNCACO | sample_1 | solution | sample_conditions_1 |
| HNHB | sample_1 | solution | sample_conditions_1 |
Software:
Ansig vany, Kraulis - chemical shift assignment
AutoDep v4.3, PDBe - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| UNP | SNF5_HUMAN |
| PDB | |
| DBJ | BAA95062 BAB12427 BAC36045 BAE90809 BAG61393 |
| EMBL | CAA09758 CAA09760 CAA09761 CAA76639 CAG30467 |
| GB | AAA81905 AAB34227 AAH25163 AAI33573 AAO59167 |
| REF | NP_001035647 NP_001270057 NP_003064 NP_035548 XP_001169712 |
| SP | Q12824 Q5BIN2 Q9Z0H3 |
| TPG | DAA20401 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts