BMRB Entry 26550
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26550
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Title: Sequence-specific solid-state NMR assignments of the mouse ASC PYRIN domain in its filament form PubMed: 26464513
Deposition date: 2015-03-31 Original release date: 2015-10-12
Authors: Ravotti, Francesco; Sborgi, Lorenzo; Cadalbert, Riccardo; Huber, Matthias; Hiller, Sebastian; Meier, Beat; Boeckmann, Anja
Citation: Sborgi, Lorenzo; Ravotti, Francesco; Dandey, Venkata; Dick, Mathias; Mazur, Adam; Reckel, Sina; Chami, Mohamed; Scherer, Sebastian; Huber, Matthias; Bockmann, Anja; Egelman, Edward; Stahlberg, Henning; Broz, Petr; Meier, Beat; Hiller, Sebastian. "Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy" Proc. Natl. Acad. Sci. U. S. A. 112, 13237-13242 (2015).
Assembly members:
Mouse_ASC_PYD, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Mouse_ASC_PYD: MGRARDAILDALENLSGDEL
KKFKMKLLTVQLREGYGRIP
RGALLQMDAIDLTDKLVSYY
LESYGLELTMTVLRDMGLQE
LAEQLQTTKEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 395 |
| 15N chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mouse ASC PYD | 1 |
Entities:
Entity 1, Mouse ASC PYD 91 residues - Formula weight is not available
| 1 | MET | GLY | ARG | ALA | ARG | ASP | ALA | ILE | LEU | ASP | ||||
| 2 | ALA | LEU | GLU | ASN | LEU | SER | GLY | ASP | GLU | LEU | ||||
| 3 | LYS | LYS | PHE | LYS | MET | LYS | LEU | LEU | THR | VAL | ||||
| 4 | GLN | LEU | ARG | GLU | GLY | TYR | GLY | ARG | ILE | PRO | ||||
| 5 | ARG | GLY | ALA | LEU | LEU | GLN | MET | ASP | ALA | ILE | ||||
| 6 | ASP | LEU | THR | ASP | LYS | LEU | VAL | SER | TYR | TYR | ||||
| 7 | LEU | GLU | SER | TYR | GLY | LEU | GLU | LEU | THR | MET | ||||
| 8 | THR | VAL | LEU | ARG | ASP | MET | GLY | LEU | GLN | GLU | ||||
| 9 | LEU | ALA | GLU | GLN | LEU | GLN | THR | THR | LYS | GLU | ||||
| 10 | GLU |
Samples:
sample_1: Mouse ASC PYD, [U-100% 13C; U-100% 15N], 20 mg
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NCACX | sample_1 | isotropic | sample_conditions_1 |
| NCOCX | sample_1 | isotropic | sample_conditions_1 |
| NCACB | sample_1 | isotropic | sample_conditions_1 |
| CANCO | sample_1 | isotropic | sample_conditions_1 |
| CCC | sample_1 | isotropic | sample_conditions_1 |
| NCA | sample_1 | isotropic | sample_conditions_1 |
| NCO | sample_1 | isotropic | sample_conditions_1 |
| 20msDARR | sample_1 | isotropic | sample_conditions_1 |
| N(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| CAN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| N(CA)CBCX | sample_1 | isotropic | sample_conditions_1 |
| 100msPDSD | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin, CCPN - collection, data analysis, processing
NMR spectrometers:
- Bruker Avance 850 MHz