BMRB Entry 25900
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25900
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Title: NRAS Isoform 5 PubMed: 26947772
Deposition date: 2015-11-13 Original release date: 2016-03-21
Authors: Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William
Citation: Markowitz, Joseph; Mal, Tapas; Yuan, Chunhua; Courtney, Nicholas; Patel, Mitra; Stiff, Andrew; Blachly, James; Walker, Christopher; Eisfeld, Ann-Kathrin; de la Chapelle, Albert; Carson, William. "Structural Characterization of NRAS Isoform 5" Protein Sci. 25, 1069-1074 (2016).
Assembly members:
entity, polymer, 20 residues, 2120.511 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: MTEYKLVVVGAGGVGKSHVW
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 150 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 20 residues - 2120.511 Da.
| 1 | MET | THR | GLU | TYR | LYS | LEU | VAL | VAL | VAL | GLY | |
| 2 | ALA | GLY | GLY | VAL | GLY | LYS | SER | HIS | VAL | TRP |
Samples:
sample_1: TFE, [U-99% 2H], 56%; entity 1 mM; sodium azide 10 uM; sodium phosphate 4 mM; EDTA, [U-99% 2H], 50 uM
sample_conditions_1: ionic strength: 0.004 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-1H HSQC-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-1H HSQC-NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz