BMRB Entry 25649

Assembly:

Entities:

Entity 1, entity_1 86 residues - 9291.632 Da.

Residues 1-5 (GAIAM) are non-native cloning artifacts. Residues 6-84 are human Brd4 ET domain (600-678) Residues 85-86 (TR) are non native cloning artifacts.

Entity 2, entity_2 17 residues - 2157.567 Da.

residues 1-17 are MLV Integrase C-term EBM(389-405)

Samples:

U13C-15NBrd4_MLV_in_H2O: Brd4 ET, [U-99% 13C; U-99% 15N], 0.4 – 0.8 mM; MLV IN EBM0.4 – 0.8 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; sodium azide 0.002 % v/v; DSS 0.5 mM; DTT, [U-2H], 2 mM; H2O 90%; D2O 10%

U13C-15NBrd4_MLV_in_D2O: Brd4 ET, [U-99% 13C; U-99% 15N], 0.4 – 0.8 mM; MLV IN EBM0.4 – 0.8 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; sodium azide 0.002 % v/v; DSS 0.5 mM; DTT, [U-2H], 2 mM; D2O 100%

U15NBrd4_MLV_in_H2O: Brd4 ET, [U-99% 15N], 0.4 – 0.8 mM; MLV IN EBM0.4 – 0.8 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; sodium azide 0.002 % v/v; DSS 0.5 mM; DTT, [U-2H], 2 mM; H2O 90%; D2O 10%

U15NBrd4_free: Brd4 ET, [U-99% 15N], 0.4 – 0.8 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; sodium azide 0.002 % v/v; DSS 0.5 mM; DTT, [U-2H], 2 mM; H2O 90%; D2O 10%

MLV_IN_EBM_in_H2O: MLV IN EBM 1 mM; DSS 0.5 mM; sodium azide 0.002 % v/v; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

xwinnmr, Bruker Biospin - collection

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ v8.1.17, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

TALOS-N, Cornilescu, Delaglio and Bax - data analysis

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

• Bruker Avance III HD Ultrashield 600 MHz
• Bruker Avance III HD Ascend 700 MHz
• Bruker Avance III HD 800 MHz