BMRB Entry 25610
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25610
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Title: NMR solution structure of the pheromone Ep-1 from Euplotes petzi PubMed: 27455370
Deposition date: 2015-05-14 Original release date: 2016-04-25
Authors: Pedrini, Bill; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo
Citation: Pedrini, Bill; Suter-Stahel, Thea; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo. "Molecular Structures and Coding Genes of the Water-Borne Protein Pheromones of Euplotes petzi, an Early Diverging Polar Species of Euplotes" J. Eukaryot. Microbiol. 64, 164-172 (2017).
Assembly members:
Ep-1, polymer, 32 residues, 3180.605 Da.
Natural source: Common Name: ciliates Taxonomy ID: 1156992 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes petzi
Experimental source: Production method: purified from the natural source Host organism: Euplotes petzi
Entity Sequences (FASTA):
Ep-1: SCGSECAPEPDCWGCCLVQC
APSICAGWCGGS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 163 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 32 residues - 3180.605 Da.
| 1 | SER | CYS | GLY | SER | GLU | CYS | ALA | PRO | GLU | PRO | ||||
| 2 | ASP | CYS | TRP | GLY | CYS | CYS | LEU | VAL | GLN | CYS | ||||
| 3 | ALA | PRO | SER | ILE | CYS | ALA | GLY | TRP | CYS | GLY | ||||
| 4 | GLY | SER |
Samples:
sample_1: Ep-1 0.25 ± 0.05 mM; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: ionic strength: 0.06 M; pH: 6.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al., Keller and Wuthrich - chemical shift assignment, refinement
ATNOS, Guentert, P, Herrmann, T, Wuethrich, K - peak picking
CANDID, Guentert, P, Herrmann, T, Wuethrich, K - peak assignment, upper distance constraint determination
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz