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Biological Magnetic Resonance Data Bank


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BMRB Entry 25210

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25210
MolProbity Validation Chart

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Title: Shotgun proteolysis: A practical application

Deposition date: 2014-09-09 Original release date: 2014-09-15

Authors: Allen, Mark; Bycroft, M.; Freund, S.; Christ, D.

Citation: Allen, Mark; Bycroft, M.; Freund, S.; Christ, D.. "Shotgun proteolysis: A practical application"  To Be Published ., .-..

Assembly members:
PUTATIVE_MEMBRANE_PROTEIN_IGAA_HOMOLOG, polymer, 120 residues, 13419.934 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PUTATIVE_MEMBRANE_PROTEIN_IGAA_HOMOLOG: MAFADAQTRKLTPEERSAVE NYLESLTQVLQVPGPTGASA APISLALNAESNNVMMLTHA ITRYGISTDDPNKWRYYLDS VEVHLPPFWEQYINDENTVE LIHTDSLPLVISLNGHTLQE

Data sets:
Data typeCount
1H chemical shifts853
13C chemical shifts524
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG1

Entities:

Entity 1, PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG 120 residues - 13419.934 Da.

1   METALAPHEALAASPALAGLNTHRARGLYS
2   LEUTHRPROGLUGLUARGSERALAVALGLU
3   ASNTYRLEUGLUSERLEUTHRGLNVALLEU
4   GLNVALPROGLYPROTHRGLYALASERALA
5   ALAPROILESERLEUALALEUASNALAGLU
6   SERASNASNVALMETMETLEUTHRHISALA
7   ILETHRARGTYRGLYILESERTHRASPASP
8   PROASNLYSTRPARGTYRTYRLEUASPSER
9   VALGLUVALHISLEUPROPROPHETRPGLU
10   GLNTYRILEASNASPGLUASNTHRVALGLU
11   LEUILEHISTHRASPSERLEUPROLEUVAL
12   ILESERLEUASNGLYHISTHRLEUGLNGLU

Samples:

sample_1: PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG, [U-13C; U-15N], 1.5 mM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 120.000 mM; pH: 6.500; pressure: 1 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1solutionsample_conditions_1
1H-13C HSQCsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HN(CA)COsample_1solutionsample_conditions_1
HNHBsample_1solutionsample_conditions_1
1H-NOESYsample_1solutionsample_conditions_1
1H-DQF-COSYsample_1solutionsample_conditions_1
1H-TOCSYsample_1solutionsample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP IGAA_ECOLI
PDB
DBJ BAB37663 BAE77893 BAG79184 BAI27656 BAI32826
EMBL CAP77845 CAQ33719 CAR00337 CAR04998 CAR10194
GB AAA58195 AAC76423 AAG58498 AAN44877 AAN82607
REF NP_312267 NP_417857 NP_709170 WP_000104248 WP_000104249
SP P45800 P58720

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