BMRB Entry 19660
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19660
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Title: Structure determination of the salamander courtship pheromone Plethodontid Modulating Factor PubMed: 24849290
Deposition date: 2013-12-05 Original release date: 2014-01-02
Authors: Wilburn, Damien; Bowen, Kathleen; Doty, Kari; Arumugam, Sengodagounder; Lane, Andrew; Feldhoff, Pamela; Feldhoff, Richard
Citation: Wilburn, Damien; Bowen, Kathleen; Doty, Kari; Arumugam, Sengodagounder; Lane, Andrew; Feldhoff, Pamela; Feldhoff, Richard. "Structural Insights into the Evolution of a Sexy Protein: Novel Topology and Restricted Backbone Flexibility in a Hypervariable Pheromone from the Red-Legged Salamander, Plethodon shermani." PLoS ONE 9, e96975-e96975 (2014).
Assembly members:
rPMF-G, polymer, 57 residues, 6267.869 Da.
Natural source: Common Name: red-legged salamander Taxonomy ID: 263671 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Plethodon shermani
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
rPMF-G: LQCNTLDGGTEECIPGIYNV
CVHYKSEDEEYKSCGIQEEC
EDAEGATVLCCPEDLCN
- assigned_chemical_shifts
- H_exch_rates
| Data type | Count |
| 13C chemical shifts | 171 |
| 15N chemical shifts | 56 |
| 1H chemical shifts | 352 |
| H exchange rates | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 57 residues - 6267.869 Da.
| 1 | LEU | GLN | CYS | ASN | THR | LEU | ASP | GLY | GLY | THR | ||||
| 2 | GLU | GLU | CYS | ILE | PRO | GLY | ILE | TYR | ASN | VAL | ||||
| 3 | CYS | VAL | HIS | TYR | LYS | SER | GLU | ASP | GLU | GLU | ||||
| 4 | TYR | LYS | SER | CYS | GLY | ILE | GLN | GLU | GLU | CYS | ||||
| 5 | GLU | ASP | ALA | GLU | GLY | ALA | THR | VAL | LEU | CYS | ||||
| 6 | CYS | PRO | GLU | ASP | LEU | CYS | ASN |
Samples:
sample_1: rPMF-G, [U-98% 15N], 2.2 mM; KCl 50 mM; NaPO4 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection
TALOS, Cornilescu, Delaglio and Bax - data analysis
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts