BMRB Entry 19600
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19600
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: CEH37 Homeodomain PubMed: 24361878
Deposition date: 2013-11-04 Original release date: 2014-02-11
Authors: Moon, Sunjin; Lee, Weontae
Citation: Moon, Sunjin; Lee, Yong Woo; Kim, Woo Taek; Lee, Weontae. "Solution structure of CEH-37 homeodomain of the nematode Caenorhabditis elegans." Biochem. Biophys. Res. Commun. 443, 370-375 (2014).
Assembly members:
CEH-37_Homeodomain, polymer, 68 residues, 8511.745 Da.
Natural source: Common Name: nematode Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CEH-37_Homeodomain: GPRKNRRERTTYSRQQLEIL
ETLFNETQYPDVFARERVAD
QIRLQESRIQVWFKNRRAKY
RLQEKQKP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 199 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 431 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CEH37 Homeodomain | 1 |
Entities:
Entity 1, CEH37 Homeodomain 68 residues - 8511.745 Da.
| 1 | GLY | PRO | ARG | LYS | ASN | ARG | ARG | GLU | ARG | THR | ||||
| 2 | THR | TYR | SER | ARG | GLN | GLN | LEU | GLU | ILE | LEU | ||||
| 3 | GLU | THR | LEU | PHE | ASN | GLU | THR | GLN | TYR | PRO | ||||
| 4 | ASP | VAL | PHE | ALA | ARG | GLU | ARG | VAL | ALA | ASP | ||||
| 5 | GLN | ILE | ARG | LEU | GLN | GLU | SER | ARG | ILE | GLN | ||||
| 6 | VAL | TRP | PHE | LYS | ASN | ARG | ARG | ALA | LYS | TYR | ||||
| 7 | ARG | LEU | GLN | GLU | LYS | GLN | LYS | PRO |
Samples:
sample_1: CEH-37 Homeodomain, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%
conditions_1: pH: 7.5; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | conditions_1 |
| 3D HNCACB | sample_1 | isotropic | conditions_1 |
| 3D HNCO | sample_1 | isotropic | conditions_1 |
| 3D HNCA | sample_1 | isotropic | conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAB02825 CDX47432 CDX47433 |
| GB | ACV72913 ACV72914 ACV72915 ACV72916 ACV72917 |
| REF | NP_001294799 NP_001294800 NP_510366 XP_003102040 |
| SP | Q93356 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts