BMRB Entry 19568
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19568
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Title: assignment of the transmembrane domain of insulin receptor in detergent micelles PubMed: 24440425
Deposition date: 2013-10-19 Original release date: 2014-02-12
Authors: Li, Qingxin; Wong, Yinglei; Kang, Congbao
Citation: Li, Qingxin; Wong, Ying Lei; Kang, CongBao. "Solution structure of the transmembrane domain of the insulin receptor in detergent micelles." Biochim. Biophys. Acta 1838, 1313-1321 (2014).
Assembly members:
ISU, polymer, 57 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ISU: MTYFYVTDYLDVPSNIAKII
IGPLIFVFLFSVVIGSIYLF
LRKRQPDGPLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 136 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 98 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ISUR | 1 |
Entities:
Entity 1, ISUR 57 residues - Formula weight is not available
| 1 | MET | THR | TYR | PHE | TYR | VAL | THR | ASP | TYR | LEU | ||||
| 2 | ASP | VAL | PRO | SER | ASN | ILE | ALA | LYS | ILE | ILE | ||||
| 3 | ILE | GLY | PRO | LEU | ILE | PHE | VAL | PHE | LEU | PHE | ||||
| 4 | SER | VAL | VAL | ILE | GLY | SER | ILE | TYR | LEU | PHE | ||||
| 5 | LEU | ARG | LYS | ARG | GLN | PRO | ASP | GLY | PRO | LEU | ||||
| 6 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: ISU, [U-100% 15N], 0.2 – 0.4 mM; ISU, [U-100% 13C; U-100% 15N], 0.2 – 0.4 mM; sodium phosphate 20 mM; DPC 2%; DTT 1 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v4.1, Johnson, One Moon Scientific - data analysis
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92098 |
| EMBL | CAA26096 |
| GB | AAA39318 AAA41441 AAA59174 AAA59176 AAA59177 |
| PRF | 1104300A |
| REF | NP_000199 NP_001073285 NP_034698 NP_058767 XP_001094337 |
| SP | P06213 P15127 P15208 |
| TPG | DAA27957 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts