BMRB Entry 19526
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19526
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Title: Solution NMR structure of SLED domain of Scml2 PubMed: 24727478
Deposition date: 2013-09-24 Original release date: 2014-04-22
Authors: Bezsonova, Irina
Citation: Bezsonova, Irina. "Solution NMR Structure of the DNA-binding Domain from Scml2 (Sex Comb onMidleg-like 2)" J. Biol. Chem. ., .-. (2014).
Assembly members:
SLED_Scml2, polymer, 119 residues, 13112.994 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SLED_Scml2: GSHMMSTVCVYVNKHGNFGP
HLDPKRIQQLPDHFGPGPVN
VVLRRIVQACVDCALETKTV
FGYLKPDNRGGEVITASFDG
ETHSIQLPPVNSASFALRFL
ENFCHSLQCDNLLSSQPFS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 468 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 759 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SLED domain of Scml2 | 1 |
Entities:
Entity 1, SLED domain of Scml2 119 residues - 13112.994 Da.
| 1 | GLY | SER | HIS | MET | MET | SER | THR | VAL | CYS | VAL | ||||
| 2 | TYR | VAL | ASN | LYS | HIS | GLY | ASN | PHE | GLY | PRO | ||||
| 3 | HIS | LEU | ASP | PRO | LYS | ARG | ILE | GLN | GLN | LEU | ||||
| 4 | PRO | ASP | HIS | PHE | GLY | PRO | GLY | PRO | VAL | ASN | ||||
| 5 | VAL | VAL | LEU | ARG | ARG | ILE | VAL | GLN | ALA | CYS | ||||
| 6 | VAL | ASP | CYS | ALA | LEU | GLU | THR | LYS | THR | VAL | ||||
| 7 | PHE | GLY | TYR | LEU | LYS | PRO | ASP | ASN | ARG | GLY | ||||
| 8 | GLY | GLU | VAL | ILE | THR | ALA | SER | PHE | ASP | GLY | ||||
| 9 | GLU | THR | HIS | SER | ILE | GLN | LEU | PRO | PRO | VAL | ||||
| 10 | ASN | SER | ALA | SER | PHE | ALA | LEU | ARG | PHE | LEU | ||||
| 11 | GLU | ASN | PHE | CYS | HIS | SER | LEU | GLN | CYS | ASP | ||||
| 12 | ASN | LEU | LEU | SER | SER | GLN | PRO | PHE | SER |
Samples:
sample_1: SLED_Scml2, [U-100% 13C; U-100% 15N], 0.3 – 1.0 mM; HEPES 50 mM; NaCl 250 mM; DTT 2 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 250 mM; pH: 7.2; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG61234 BAG64181 |
| EMBL | CAB38943 |
| GB | AAH51913 AAH64617 ABM54391 ABM67362 EAW98937 |
| REF | NP_001248288 NP_006080 XP_003819585 XP_003924181 XP_004063918 |
| SP | Q9UQR0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts