BMRB Entry 19437
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19437
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Title: Membrane induced structure of the mammalian tachykinin neuropeptide gamma PubMed: 15522780
Deposition date: 2013-08-19 Original release date: 2013-09-23
Authors: Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha
Citation: Chandrashekar, Indu; Ganjiwale, Anjali; Cowsik, Sudha. "Membrane-induced structure of the mammalian tachykinin neuropeptide gamma" J. Struct. Biol. 148, 315-325 (2004).
Assembly members:
NPgamma, polymer, 21 residues, 4637.265 Da.
5-ACETYLAMINO-4-AMINO-6-(PHENETHYL-PROPYL-CARBAMOYL)-5,6-DIHYDRO-4H-PYRAN-2-CARBOXYLIC ACID, non-polymer, 389.445 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
NPgamma: DAGHGQISHKRHKTDSFVGL
M
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mammalian tachykinin neuropeptide gamma | 1 |
| 2 | DPC | 2 |
Entities:
Entity 1, mammalian tachykinin neuropeptide gamma 21 residues - 4637.265 Da.
| 1 | ASP | ALA | GLY | HIS | GLY | GLN | ILE | SER | HIS | LYS | ||||
| 2 | ARG | HIS | LYS | THR | ASP | SER | PHE | VAL | GLY | LEU | ||||
| 3 | MET |
Entity 2, DPC - C20 H27 N3 O5 - 389.445 Da.
| 1 | DPC |
Samples:
sample_1: entity 4 mg; DPC, [U-100% 2H], 250 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 2.4; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - data analysis, processing
DIANA, Guntert, Braun and Wuthrich - refinement, structure solution
NMR spectrometers:
- Bruker DRX 500 MHz